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Plasmid#197288PurposeConjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transferDepositorInsertI-SceI Endonuclease
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB151
Plasmid#101666PurposeOpto-T7RNAP*(563-F1)DepositorInsertOpto-T7RNAP*(563-F1)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1E
Plasmid#160442PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRB035
Plasmid#181944PurposeaTc-inducible expression of mNeonGreenDepositorInsertmNeonGreen; tetR (AX04_RS23485 Synthetic)
UseTagsExpressionBacterialMutationPromotermNG-PLtetO-1; tetR-J23106Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAX-DEST
Plasmid#37631DepositorTypeEmpty backboneUseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGGY001
Plasmid#48866PurposeEmpty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.DepositorTypeEmpty backboneUseGolden gate compatible plant transformation vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGY003
Plasmid#48867PurposeEmpty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.DepositorTypeEmpty backboneUseGolden gate compatible plant transformation vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH674_pTech_PlldR-HHRz-supP
Plasmid#163728PurposeLactate-inducible expression of supP, preceded by a hammerhead ribozymeDepositorInserthhrz-supP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTOX3
Plasmid#127448PurposeEncodes Tse2 toxin. CAM-RDepositorInserttse2 toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR3-IR3-Bxb1-CadR-pZa
Plasmid#204686PurposeCadmium responsive genetic circuitDepositorInsertBxb1 (Bxb1p45 Synthetic)
UseSynthetic BiologyTagsCadRExpressionBacterialMutationPromoterInducible, HspR is its repressorAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasX1
Plasmid#87685Purposep15A plasmid expressing CasX locus from DeltaproteobacteriaDepositorInsertCasX1
UseTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB152
Plasmid#101663PurposeOpto-T7RNAP*(563-F2)DepositorInsertOpto-T7RNAP*(563-F2)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GFPM2
Plasmid#102791PurposeExpresses GFP under WT T7 promoterDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Magenta
Plasmid#160446PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Magenta
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Violet
Plasmid#160447PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Violet
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB203
Plasmid#101675PurposeOpto-T7RNAP(563-F1)DepositorInsertOpto-T7RNAP(563-F1)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBsaBM3
Plasmid#85102PurposeP450 BM3 variant (A74G/C773S/C810S)DepositorInsertP450 BM3
UseCloning vectorTagsExpressionMutationA74G/C773S/C810SPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSM040
Plasmid#128294Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTOX1
Plasmid#127446PurposeEncodes YhaV toxin. CAM-RDepositorInsertyhaV toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Yellow
Plasmid#160443PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Yellow
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only