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Showing: 2221 - 2240 of 8125 results

  1. pX330-PITCh-ACTB

    Plasmid
    #207748
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    sgRNA Targeting N-terminus of ACTB (ACTB Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207748-plasmid-map-sequence-id-414798

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  2. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Tags
    Expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  4. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

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  5. PXL

    Plasmid
    #75349
    Purpose
    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75349-plasmid-map-sequence-id-266810

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  6. BPK2301

    Plasmid
    #65778
    Purpose
    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    St1Cas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65778-plasmid-map-sequence-id-112652

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  8. pHDR-USR-eGFP

    Plasmid
    #208830
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    208830-plasmid-map-sequence-id-414896

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  14. pX335-EN475

    Plasmid
    #86231
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86231-plasmid-map-sequence-id-177755

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  18. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86232-plasmid-map-sequence-id-177242

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Showing: 2221 - 2240 of 8125 results