-
Plasmid#58256Purposebacterial expression of human PITP alphaDepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-Actin-7
Plasmid#57120PurposeLocalization: Actin, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertActin (ACTB Human)
UseTagsmPA-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3
Plasmid#112005PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV5 and AAV9Insertaxon-GCaMP6s-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP2
Plasmid#79874PurposeBacillus subtilis sgRNA expression vector; integrates into amyEDepositorInsertsgRNA RR1
UseCRISPRTagsExpressionBacterialMutationPromotervegAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA331Bb
Plasmid#78269PurposepSEVA331 with Biobrick multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
BECLIN-HA WT
Plasmid#46993PurposeExpresses Beclin1 in mammalian cellsDepositorInsertbecn1 (Becn1 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pX330-PITCh-GOLGA2
Plasmid#207791PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPar2
Plasmid#53228Purposemammalian expression of human Par2DepositorInsertPar2 (F2RL1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO_EGFP-GFE3
Plasmid#79871PurposeFor generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.DepositorInsertGFP-GFE3
UseAAV, Cre/Lox, and Synthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE GS-gRNA (hSyn-Cre) mTubb3
Plasmid#200369PurposePositive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)DepositorInsertMouse Tubb3 gRNA and Cre
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV10-hTel2
Plasmid#30214DepositorInserttelomere maintenance 2 (TELO2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
ANO6-Plvx-mCherry-c1
Plasmid#62554PurposeFull length mouse ANO6 tagged with mCherry on the C-terminusDepositorInsertmouse ANO6 (Ano6 Mouse)
UseLentiviralTagsmCherryExpressionMutationPromoterCMVAvailable sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRE reporter backbone
Plasmid#158678PurposeThis plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberryDepositorTypeEmpty backboneUseLentiviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC001 - huLshC2C2-MBP for bacterial expression
Plasmid#79150PurposeExpresses human codon-optimized LshC2c2 for purification in E. coliDepositorInsertC2c2
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-ACTB
Plasmid#207748PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of ACTB (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs
Plasmid#86828PurposeExpression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequenceDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Actin-C-18
Plasmid#54967PurposeLocalization: Actin, Excitation: 587, Emission: 610DepositorInsertActin (ACTB Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
iRFP-H2A
Plasmid#158691PurposeThis plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markersDepositorInsertiRFP670
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Geminin-mStrawberry reporter
Plasmid#158690PurposeThis plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only