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pCMV-dLbCpf1-BE-EE

Plasmid

#154147

Purpose

Expresses dLbCpf1-BE-EE(dLbCas12a-BE-EE) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-EE (dCas12a-BE-EE)

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(R126E+R132E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YE1

Plasmid

#154145

Purpose

Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE1 (dCas12a-BE-YE1)

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ269A2

Plasmid

#164714

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265

Plasmid

#164712

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YE2

Plasmid

#154146

Purpose

Expresses dLbCpf1-BE-YE2(dLbCas12a-BE-YE2) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE2 (dCas12a-BE-YE2)

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R132E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YEE

Plasmid

#154148

Purpose

Expresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YEE (dCas12a-BE-YEE)

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-CK2beta 6KR (pAB1016)

Plasmid

#37361

Depositor

David Litchfield

Article

French et al J Biol Chem. 2007 Oct 5;282(40):29667-77

Insert

CK2beta 6KR (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K33R, K139R, K177R, K191R, K208R, K212R

Promoter

CMV-F

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
miniCGBE1 (pBM1219)

Plasmid

#140253

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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miniCGBE1-NG (pBM1182)

Plasmid

#140257

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)_NG-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET-His6MBP-A3A-Gly6His6

Plasmid

#187822

Purpose

Expression human APOBEC3A in E.coli.

Depositor

Hao Wu

Article

Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

Insert

APOBEC3A (APOBEC3A Human)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

none

Promoter

T7 promoter (on the backbone)

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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miniCGBE1-VRQR (pBM1173)

Plasmid

#140255

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)_VRQR-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

Plasmid

#109428

Purpose

A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry T2A GFP

Use

Lentiviral

Tags

Expression

Mutation

Inserted 43 base pairs into mCherry to create rep…

Promoter

CMV

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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SECURE BE4max(R33A) (pBM608)

Plasmid

#140251

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET42b-BE3

Plasmid

#87437

Purpose

Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HF-BE3

Plasmid

#87439

Purpose

Expresses HF-BE3 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Use

Tags

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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CGBE1-VRQR (pBM1192)

Plasmid

#140254

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_VRQR-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pZW12 (CK2beta)

Plasmid

#27088

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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