...capability of the seven human APOBEC3 proteins, finding that APOBEC3A and APOBEC3bctd are the most efficient ...editing by a APOBEC-Cas9n-UGI complex. So how exactly does this work? Figure 1: APOBEC- and Cas9-mediated...that have been successfully edited with APOBEC3A and APOBEC3B (St. Martin et al., 2018). TLDR: The ACE...to monitor APOBEC-Cas-9 mediated editing. eGFP reporters for single base editing by APOBEC-Cas9 Though...reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated editing in real time The Harris...Harris lab created ACE, a reporter that monitors APOBEC (a family of cytidine deaminase proteins) and Cas9... action. Image from St. Martin et al., 2018. APOBEC-Cas9n-UGI targets its preferred trinucleotide motifs...

...formation of atherosclerotic plaques. ApoB bound lipoproteins (ApoB-LPs) are indirectly measured through...great model for high throughput study of ApoB-LPs because their ApoB homologes are functionally conserved,...endogenous ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using...PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by Leah Schwiesow...lipoproteins that accumulate within the vascular wall. ApoB, the main structural component of atherogenic lipoproteins... However, size distribution and concentration of ApoB-LPs has been shown to have a strong correlation ...cardiovascular disease and indirect measurement of ApoB-LPs through triglyceride/cholesterol does not provide...

...Reference CBE SpCas9 rAPOBEC1 NGG 6 SpCas9 Target-AID (PmCDA1) NGG 5 SpCas9-VQR rAPOBEC1 NGA 8 SaCas9-... plant systems. Target-AID based (CBE-2)-  and rAPOBEC based (CBE-1)-  CBEs are successful base editors...rice, tomato, wheat, and potato. Target-AID and rAPOBEC based CBEs are known to deaminate cytosine placed...closer to the PAM site? CBE developed using the hAPOBEC3 deaminase can edit in a stretched window ranging...SaCas9-KKH rAPOBEC1 NNNRRT 8 ABE SpCas9 TadA-TadA* NGG 9-12 SpCas9-VQR TadA-TadA* NGA 8 SaCas9 TadA-TadA...editing in plants using a fusion of nCas9 and human APOBEC3A." Nature biotechnology (2018). PubMed PMID: 30272679...

... like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and Cas9..., the Harris lab showed that human APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are...abundance, and mCherry fluorescence is activated when APOBEC-Cas9n-UGI-mediated activity edits out a frame-shift... efficient than the BE3 plasmid containing rat APOBEC1. Finally, they show that the ACE reporter can also...

...variants with other deaminases: AID, CDA1, and APOBEC3G (Komor et al., 2017). CDA1-BE3 and AID-BE3 edited...Cs following a G more efficiently than BE3, but APOBEC3G displayed less predictable sequence preferences...editing product purity. The lab also extended the APOBEC1-Cas9n and Cas9n-UGI linkers to improve product ...editor with more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly more effective...Komor et al., 2017 BE4 and BE4-Gam; AID, CDA1 and APOBEC3G BE3 variants   A second copy of UGI improves product...low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing of Cs preceded by Gs...

...found that a cytosine base editor (CBE) with rat APOBEC1 causes genome-wide deamination of RNA cytosines...engineered two CBE variants with mutations in the rat APOBEC1 that decreases the number of off-target RNA edits...

...rate of off-target genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE adenine base editor...

...Chen et al., 2018). Using the cytidine deaminase, APOBEC1 and a nickase version of Cas9 they were effectively...

...dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus ...

...nickase (Cas9n) or dCas9 to a cytidine deaminase like APOBEC. Within the region of single-stranded DNA created...

...nickase (Cas9n) or dCas9 to a cytidine deaminase like APOBEC. Within the region of single-stranded DNA created...