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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,273 results for: LEA

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Showing: 2161 - 2180 of 12273 results

  3. pSL690

    Plasmid
    #47753
    Purpose
    Expresses dCas9-VP64 fusion
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    47753-plasmid-map-sequence-id-68212

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  12. pET28-iLOVf

    Plasmid
    #63723
    Purpose
    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    TEE, iLOV, 6xHis, thrombin cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    63723-plasmid-map-sequence-id-110780

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  13. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid
    #51006
    Purpose
    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-cerFP
    Use
    Lentiviral
    Tags
    histone 2B
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51006-plasmid-map-sequence-id-77912

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  14. LVXN-Neo-NSD2-E1099K

    Plasmid
    #86011
    Purpose
    express NSD2 E1099K mutant in mammalian cells
    Depositor
    Darrin Stuart
    Publication
    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NSD2 E1099K mutant (NSD2 Human)
    Tags
    Flag tag  D Y K D D D D K
    Expression
    Mammalian
    Mutation
    E1099K; K1002R (please see depositor comments bel…
    Promoter
    CMV
    Available since
    Jan. 13, 2017
    Availability
    Academic Institutions and Nonprofits only

  15. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    181743-plasmid-map-sequence-id-354675

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  17. pCAG roGFP2-mito

    Plasmid
    #168509
    Purpose
    Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential
    Depositor
    Franck Polleux
    Publication
    Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mito-roGFP2
    Tags
    roGFP2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    168509-plasmid-map-sequence-id-329887

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  18. CBX4

    Plasmid
    #25237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX4_02 (CBX4 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
Showing: 2161 - 2180 of 12273 results