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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,061 results for: LEA

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Showing: 2161 - 2180 of 14061 results

  1. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Publication
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLtetO, native, native (respectively)
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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  4. pAAV-Ef1a-fDIO EYFP (AAV8)

    Viral prep
    #55641-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Available since
    Dec. 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  9. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  11. GEF-H1 FLARE212.pa

    Plasmid
    #134930
    Purpose
    GEF-H1 biosensor that does not have GEF activity.
    Depositor
    Klaus Hahn
    Publication
    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    Tags
    None.
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and T7
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    134930-plasmid-map-sequence-id-271271

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  13. pcDNA3-StagRFP

    Plasmid
    #173014
    Purpose
    Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.
    Depositor
    Jin Zhang
    Publication
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    StagRFP
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173014-plasmid-map-sequence-id-343313

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  16. pcDNA-RanWT-mRFP1-polyA

    Plasmid
    #59750
    Purpose
    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.
    Depositor
    Yi Zhang
    Publication
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ran-mRFP1
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Sept. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59750-plasmid-map-sequence-id-94259

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  18. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TrxRFP1 and nuclear localization sequence
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98998-plasmid-map-sequence-id-190366

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  19. pcDNA3-PM-CaNAR2

    Plasmid
    #64730
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-CaNAR2
    Tags
    Cerulean3, N-terminal targeting sequence from Lyn…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64730-plasmid-map-sequence-id-109348

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Showing: 2161 - 2180 of 14061 results