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We narrowed to 24,159 results for: LEA

Showing: 2161 - 2180 of 24159 results

  1. RFN_EGFP_80

    Plasmid
    #60072
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pCVL SFFV Sbf-Sal linker

    Plasmid
    #50434
    Purpose
    Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).
    Depositor
    Andrew Scharenberg
    Article
    Boissel et al Nucleic Acids Res. 2013 Nov 26.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50434-plasmid-map-sequence-id-75355

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  9. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194469-plasmid-map-sequence-id-384873

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  10. SUMO_MT3

    Plasmid
    #200303
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Tags
    Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200303-plasmid-map-sequence-id-394864

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  14. pCVL TAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50420
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Article
    Boissel et al Nucleic Acids Res. 2013 Nov 26.
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50420-plasmid-map-sequence-id-75338

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  15. eSrtA-MT4

    Plasmid
    #200306
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT4 (MT4 Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200306-plasmid-map-sequence-id-394863

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  16. SUMO_LPETG_MT3

    Plasmid
    #200304
    Purpose
    Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Tags
    Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200304-plasmid-map-sequence-id-394896

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Showing: 2161 - 2180 of 24159 results