We narrowed to 17,504 results for: LEA

1. pPAP_BGL1

   Plasmid

   #183735

   Purpose

   Expresses Aspergillus aculeatus β-glucosidase (BGL1)

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   β-glucosidase (AaBGL1)
   

   Expression

   Yeast

   Promoter

   AOX1 promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-TRAIL-R1

   Plasmid

   #61382

   Purpose

   Expresses HA tagged TRAIL receptor 1

   Depositor

   Francis Chan

   Article

   Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

   Insert

   TRAIL receptor 1
   

   Tags

   HA and TNF receptor leader peptide

   Expression

   Mammalian

   Mutation

   TRAIL-R1 leader peptide (aa1-23) replaced with TN…

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pMM0646

   Plasmid

   #129007

   Purpose

   Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

   Plasmid

   #45579

   Purpose

   Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani K227M
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB22

   Plasmid

   #61342

   Purpose

   Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Tags

   GS linker-AsLov2-cMyc1A NLS and PKIt NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-mCherry-Rheb(5A)

   Plasmid

   #192439

   Purpose

   Encodes mCherry-tagged Rheb lacking residues 38-42.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-Rheb(C181S) (RHEB Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Rheb amino acids 38-42 deleted.

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-mCherry-CyrCR1.miR16

   Plasmid

   #128747

   Purpose

   Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTetOne-DsRed-cNLS

   Plasmid

   #138528

   Purpose

   Inducible expression of DsRed-Express2 with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   DsRed-Express2
   

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE3Gs

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p1553 CMV-EBNA1 (delta GGA)

    Plasmid

    #26648

    Depositor

    Bill Sugden

    Article

    Aiyar et al J Biol Chem. 1998 Dec 4. 273(49):33073-8

    Insert

    Epstein-Barr Nuclear Antigen 1 (EBNA-1 Epstein-Barr Virus)
    

    Expression

    Mammalian

    Mutation

    deletion of 224 amino acids of EBNA-1 (aa101-324 …

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-AktAR2-NLS

    Plasmid

    #175012

    Purpose

    Nuclear-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDN42

    Plasmid

    #61345

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Tags

    GS linker-AsLov2-biNLS10 and PKIt NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tmem214 pmRFP-N2 (1348)

    Plasmid

    #62047

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pMM0638

    Plasmid

    #129005

    Purpose

    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tmem214 pEGFP-N2 (1072)

    Plasmid

    #62026

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-InPAkt

    Plasmid

    #181914

    Purpose

    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

    Insert

    InPAkt
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-mCherry::LANS1

    Plasmid

    #60784

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS1
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2B-mScarlet- hRec8(297-506)-mNeonGreen

    Plasmid

    #174717

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Rec8

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Rec8 (REC8 Human)
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    297-506

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF NS34a-msGFP-'S-mito

    Plasmid

    #158765

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMM0641

    Plasmid

    #129006

    Purpose

    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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