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Showing: 2121 - 2140 of 150056 results

  1. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  2. mCherry-KLC2-N-18

    Plasmid
    #62747
    Purpose
    Localization: Kinesin Light Chain , N1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    KLC2
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pAH123

    Plasmid
    #66077
    Purpose
    The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:
    Insert
    phi80 integrase
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    66077-plasmid-map-sequence-id-112400

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  6. pET22b_PstS_1

    Plasmid
    #78198
    Purpose
    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A197C (please see depositors comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78198-plasmid-map-sequence-id-146048

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  11. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Nucleocapsid
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208937-plasmid-map-sequence-id-418963

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  12. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Use
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171782-plasmid-map-sequence-id-443047

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  14. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

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  15. pAH121

    Plasmid
    #164901
    Purpose
    The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.
    Depositor
    Keith Shearwin
    Article
    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612
    Insert
    phi21 Integrase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164901-plasmid-map-sequence-id-327647

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  17. mCherry-WASH1-C-18

    Plasmid
    #55162
    Purpose
    Localization: Cytoskeleton, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    WASH1
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-hSyn-hChR2(H134R)-mCherry (AAV5)

    Viral Prep
    #26976-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 2121 - 2140 of 150056 results