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Plasmid#225877PurposeAllows for expression of recombinant antibodiesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO EYFP (AAV2)
Viral Prep#27056-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsEYFP (Cre-dependent)Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Neo)
Plasmid#89180PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 ZoAl N-1F
Plasmid#203763PurposeFor expression of N-flag tagged ZoAl R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA Ef1alpha Puro-T2A-GFP
Plasmid#111596PurposesgRNA Ef1alpha Puro-T2A-GFPDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B
Plasmid#106309PurposeThe plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.DepositorInsert6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
UseTags6xflag-3xHaloTag(MtoL)ExpressionMammalianMutationPromoterEF1Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-puro
Plasmid#45567DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsIRES-EGFP-PuroExpressionMammalianMutationPromoterPhcmv (strong human cytomegalovirus immediate ear…Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP
Plasmid#30130DepositorInsertEnhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-H2B Control Plasmid
Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAG-GFP
Plasmid#16664DepositorInserteGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3XHA-p53
Plasmid#196267PurposeMammalian expression of 3xHA tagged wild-type p53DepositorInsertp53 (Trp53 Mouse)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Cre (AAV Retrograde)
Viral Prep#55636-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-Cre (#55636). In addition to the viral particles, you will also receive purified pAAV-EF1a-Cre plasmid DNA. EF1a-driven expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-BFP (AAV8)
Viral Prep#137131-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-BFP (#137131). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-BFP plasmid DNA. EF1a-driven, Flp-dependent expression of BFP (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsBFP (Flp-dependent)Available sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Limk1
Plasmid#163414PurposeExpresses LIMK1 in mammalian cellsDepositorInsertLimk1 (Limk1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCherry.90 alpha
Plasmid#108222PurposeMammalian expression vector for mCherry fusion to human Hsp90 alphaDepositorInsertmCherry-Hsp90a (HSP90AA1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Neo
Plasmid#85139Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -