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Showing: 2101 - 2120 of 153765 results

  1. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-434022

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  5. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

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  12. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid
    #162279
    Purpose
    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN2A Variant 1, stabilized (SCN2A Human)
    Use
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp4551.
    Promoter
    CMV
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162279-plasmid-map-sequence-id-321873

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  13. CMV-SNAP-hCB1_IRES_EGFP

    Plasmid
    #223505
    Purpose
    Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag-CNR1 and EGFP (CNR1 Human, Synthetic)
    Use
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223505-plasmid-map-sequence-id-443299

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  16. pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)

    Viral Prep
    #137128-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    None
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  17. pSBtet-GH

    Plasmid
    #60498
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60498-plasmid-map-sequence-id-97549

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  18. pAAV-S5E2-Gq-P2A-dTomato (AAV9)

    Viral Prep
    #135635-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-S5E2-Gq-P2A-dTomato (#135635). In addition to the viral particles, you will also receive purified pAAV-S5E2-Gq-P2A-dTomato plasmid DNA. Expression of Gq-DREADD and dTomato (physically separate) under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Promoter
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pGEX6P1-N-FLAG

    Plasmid
    #119759
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    FLAG and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119759-plasmid-map-sequence-id-233663

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Showing: 2101 - 2120 of 153765 results