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Showing: 2081 - 2100 of 153765 results

  3. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid
    #219565
    Purpose
    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature (2024)
    Insert
    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219565-plasmid-map-sequence-id-438331

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  11. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  15. pCMV-15F11-HA-mCh

    Plasmid
    #129591
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129591-plasmid-map-sequence-id-254269

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  16. pCENPTΔC-dCas9-3xGFP

    Plasmid
    #198325
    Purpose
    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus
    Depositor
    Sarah McClelland
    Article
    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:
    Insert
    CENPTΔC (CENPT Human)
    Use
    Lentiviral
    Tags
    EGFP x 3 and dCas9
    Expression
    Mammalian
    Mutation
    truncation - encodes only amino acids 1-375
    Promoter
    CMV-TetO
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198325-plasmid-map-sequence-id-390299

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  17. mCherry-Peroxisomes-2

    Plasmid
    #54520
    Purpose
    Localization: Peroxisomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Peroximal Targeting Signal 1 (PTS1) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54520-plasmid-map-sequence-id-337369

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  20. AAV-CamKIIa-jGCaMP8m-WPRE

    Plasmid
    #176751
    Purpose
    AAV-mediated expression of GCaMP8m under the CamKIIa promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176751-plasmid-map-sequence-id-349058

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Showing: 2081 - 2100 of 153765 results