-
Plasmid#178287PurposeExpresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAR69_STRI_m(KS_MAT)_H8_pET22b
Plasmid#122849PurposeExpresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertm(KS_MAT) (Fasn Mouse)
UseTagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b
Plasmid#122850PurposeExpresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coliDepositorInsertm(KS_MAT)_mouseL_ACP (Fasn Mouse)
UseTagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-GST
Plasmid#51533PurposeExpress GST-tagged mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM6-3F2-mCherry
Plasmid#73418PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.DepositorInsertPromoter 3F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP3F2 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His-IkappaBalpha(FL)
Plasmid#44738DepositorInsertIkappaBalpga (Nfkbia Mouse)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM6-H10-mCherry
Plasmid#66531PurposeExpresses mCherry under the control of Mutant T7 Promoter H10DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - H10Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-H391Y
Plasmid#122692PurposeExpress His-tagged PKM2 (H391Y) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationH391YPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p42)
Plasmid#135941PurposeExpresses AUF1p42 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:mVenus
Plasmid#133970PurposeHeterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.DepositorInsertmVenus
UseSynthetic BiologyTagsExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-ZZ(1700-1751)
Plasmid#99334PurposeBacterial expression of mouse CBP ZZ domainDepositorInsertCBP ZZ domain (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETCON-IgG1/design_50
Plasmid#30301DepositorInsertIgG Fc Designed Binder IgG1
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-YPET pDRF1-GW
Plasmid#168063PurposeFRET fusion between ymScarletI and YPET for use in yeastDepositorInsertymScarletI-YPET
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Cdc12-Cdc11
Plasmid#131162PurposeExpression of Septin rodsDepositorInsert6his-Cdc12 + Cdc11
UseBicistronicTagsCdc11: S-Tag and Cdc12: 6hisExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-HMGS
Plasmid#108945Purposeheterologous expression of HMGS of E. faecalis in E. coliDepositorInsertHMG-CoA synthase (HMGS)
UseTagsHis6-TagExpressionBacterialMutationA110GPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
UseTagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET32a-His-FADD
Plasmid#78803PurposeTo purify His-tagged recombinant FADD proteinDepositorInsertGIG3 (FADD Human)
UseTags6x His, S-Tag, Thrombin cleavage site, and TrxA (…ExpressionBacterialMutationPromoterT7Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-HRASLS3-1-132
Plasmid#100725PurposeExpression of N-terminal region of human HRASLS3 in E.coliDepositorInsertHRASLS3 (PLAAT3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only