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Addgene

pET32a-His-FADD
(Plasmid #78803)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78803 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-32a
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GIG3
  • Species
    H. sapiens (human)
  • Entrez Gene
    FADD (a.k.a. GIG3, IMD90, MORT1)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • Thrombin cleavage site (N terminal on backbone)
    • S-Tag (N terminal on backbone)
    • TrxA (thioredoxin) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32a-His-FADD was a gift from Jaewhan Song (Addgene plasmid # 78803 ; http://n2t.net/addgene:78803 ; RRID:Addgene_78803)