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Plasmid#79870PurposeExpresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.DepositorInsertGFP-RandE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
shCDK4/6(1)
Plasmid#73554Purposevector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)DepositorInsertshRNA against CDK4 + shRNA against CDK6
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6m
Plasmid#111261PurposeFor enriched expression of GCaMP6m in axonsDepositorHas ServiceAAV9Insertaxon-GCaMP6m
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
CDK4mut(K35M)-3XFlag-pBabe
Plasmid#73559Purposevector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tagsDepositorInsertCDK4mut(K35M)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterSV40Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRTagsExpressionBacterialMutationPromoterTet promoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS
Plasmid#79067PurposeExpress owl monkey TRIMCyp in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Actin-7
Plasmid#57943PurposeLocalization: Actin, Excitation: 549, Emission: 565DepositorInsertActin (ACTB Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV rat SAPAP1
Plasmid#47940PurposeExpresses rat SAPAP1 in mammalian cellsDepositorInsertSAPAP1 (Dlgap1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
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PML-ER-pBabe
Plasmid#73556Purposevector encoding a fusion protein between PML-IV and ER (estrogen receptor)DepositorInsertPML-IV (PML Human)
UseRetroviralTagsERExpressionMammalianMutationPromoterSV40Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-rhesusTRIM5-FOS
Plasmid#79059PurposeExpress rhesus TRIM5 in mammalian cellsDepositorInsertrhesusTRIM5-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dusp6DM_S159/197A
Plasmid#67684Purpose3' entry clone with activated Dusp6 (S159/167A)DepositorInsertdusp6 (dusp6 Zebrafish)
UseTagsExpressionMutationS159A, S197APromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1
Plasmid#86816Purposemammalian expression of lncRNA LINC00673/SLNCR1DepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + codon optimized Brn3a
Plasmid#86829Purposemammalian expression of the transcription factor Brn3aDepositorInsertBrn3a (POU4F1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
RCAS-HA-b-actin
Plasmid#125834PurposeRetroviral expression of HA-tagged human beta-actinDepositorInsertBeta-actin (ACTB Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQZ083
Plasmid#106417PurposeExpress Complexin-1 fragment (1-83) in E. coliDepositorInsertcomplexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)
UseTagsGST tagExpressionBacterialMutationFragment 1-83, Codon optimizationPromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
SI16-Kit a
Plasmid#58948Purposeexpresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cellsDepositorInsertKit receptor a mouse monoclonal antibody (kita Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1
Plasmid#159166PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1
UseTagsSV40 NLSExpressionYeastMutationPromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)
Plasmid#79034PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only