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Showing: 2021 - 2040 of 2886 results

  1. mClY-N1

    Plasmid
    #90457
    Purpose
    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1
    Insert
    mClY-N1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90457-plasmid-map-sequence-id-178580

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  2. Anti-GFP [N86/8R] (Recombinant Antibody)

    Antibody
    #184171-rAb
    Purpose
    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody. Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively).
    Depositor
    James Trimmer
    Article
    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1
    Recommended Applications
    Western Blot
    Reactivity
    Synthetic
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available since
    Oct. 14, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  3. pDH5

    Plasmid
    #83775
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; his selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83775-plasmid-map-sequence-id-159997

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  5. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  6. C20m

    Plasmid
    #120961
    Purpose
    MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)
    Depositor
    Richard Murray
    Insert
    sfYFP-ssrA (has BbsI site)
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120961-plasmid-map-sequence-id-235979

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  7. pJJB1426

    Plasmid
    #218622
    Purpose
    Express tNCS to the peroxisome for toxicity assay
    Depositor
    John Dueber
    Insert
    tNCS
    Use
    Tags
    YFP-ePTS1
    Expression
    Yeast
    Mutation
    106-588
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218622-plasmid-map-sequence-id-431872

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  8. pJJB1462

    Plasmid
    #218623
    Purpose
    Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease
    Depositor
    John Dueber
    Insert
    YFP
    Use
    Tags
    ePTS1
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218623-plasmid-map-sequence-id-432996

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  9. pJJB1463

    Plasmid
    #218624
    Purpose
    Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease
    Depositor
    John Dueber
    Insert
    YFP
    Use
    Tags
    ePTS1
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218624-plasmid-map-sequence-id-432999

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  11. MSCV-Eya2

    Plasmid
    #49265
    Purpose
    retroviral expression of Eya2 and YFP
    Depositor
    Heide Ford
    Insert
    Eya2 (EYA2 Human)
    Use
    Retroviral
    Tags
    FLAG and IRES-YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pJJB1357

    Plasmid
    #218595
    Purpose
    Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1
    Depositor
    John Dueber
    Insert
    Pex11
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218595-plasmid-map-sequence-id-431861

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  13. pAM5080

    Plasmid
    #85106
    Purpose
    Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85106-plasmid-map-sequence-id-189868

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  17. Ubc-c005

    Plasmid
    #216876
    Purpose
    Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion
    Depositor
    Lari Lehtiö
    Insert
    Ubiquitin (UBC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216876-plasmid-map-sequence-id-434371

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  18. Anti-GFP [N86/38.1R] (Recombinant Antibody)

    Antibody
    #180084-rAb
    Purpose
    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody. Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively).
    Depositor
    James Trimmer
    Article
    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1
    Recommended Applications
    Immunocytochemistry, Immunohistochemistry, and Western Blot
    Reactivity
    Synthetic
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available since
    March 29, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Immunocytochemistry image by Addgene

  19. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Expression
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  20. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Use
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2021 - 2040 of 2886 results