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Plasmid#119862Purposeprecusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287CDepositorInsertD-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
UseTagsnoneExpressionBacterialMutationLeucine 244 to Cysteine, Aspartate 287 to CysteinePromoterT5Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_YFP
Plasmid#45295DepositorInsertYFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDZ645 pKAN 1x-mCherry-12xPP7 V4
Plasmid#73173PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert1x-mCherry
UseTagsExpressionMammalian and YeastMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-Foxd3 in situ
Plasmid#37098DepositorInsertFoxd3 (Foxd3 Mouse)
UseIn situ hybridization probeTagsExpressionMutationPromoterAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRL1124
Plasmid#70690Purposeprovides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.DepositorInsertsavaiM
eco47iiM
ecot22iM
UseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTEI036
Plasmid#105350PurposeSynthetic biologyDepositorInsertLexA operator & 35S min promoter
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+p35SoTMV
Plasmid#170876PurposePhytobrick - p35SoTMVDepositorInsertp35SoTMV
UseTagsExpressionPlantMutation214C>T and 229C>T to remove BbsI restrictio…PromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM1761
Plasmid#105381PurposeSynthetic biologyDepositorInsertpromoter AtDMC1 (At3G22880; 3000bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_NcVVD-LOV_X
Plasmid#131176PurposeExpresses NcVVD-LOV (Neurosporra crassa) followed by restriction sitesDepositorInsertNcVVD-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_AtPHYB-S_X
Plasmid#131183PurposeExpresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sitesDepositorInsertAtPHYB-S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
TTVTFLPTPSLILQT_epitope
Plasmid#165021PurposeExpression of CMV UL44 (38-52) fused to CD74DepositorInsertCMV UL44 (38-52)
UseLentiviralTagsCD74 at N-term; cathepsin S cleavage site (GRWHTV…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_ScPH1-S_X
Plasmid#131184PurposeExpresses ScPH1-S (Synechosystis PCC6803) followed by restriction sitesDepositorInsertScPH1-S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_RsLP-LOV_X
Plasmid#131177PurposeExpresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sitesDepositorInsertRsLP-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI
Plasmid#162801PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expressionDepositorInsertUb-BsmBI
UsePiggybacTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOG331
Plasmid#105408PurposeSynthetic biologyDepositorInsert6xHA tag fused to Twin-Strep tag
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI071
Plasmid#105376PurposeSynthetic biologyDepositorInsertpromotor CAB3 (At1g29910; 1000bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-TAX-L10-STEPtag
Plasmid#159375PurposeExpresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertTAX-L10-STEPtag
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-STEPtag-L10-TAX
Plasmid#159374PurposeExpresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertSTEPtag-L10-TAX
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK032
Plasmid#105412PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A
Plasmid#129673PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b-d570a entry …TagsExpressionPlantMutationD570A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only