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Plasmid#108865PurposeExpresses murine alpha synucleinDepositorInsertalpha synuclein (Snca Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3992 pET ubcH5
Plasmid#8652DepositorInsertUbcH5 (UBE2D1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1 pET151
Plasmid#159384PurposeExpresses human TLNRD1 in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72P)
Plasmid#62083PurposeExpression of human p53 (1-73) (72P) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72PPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-deltaPYD
Plasmid#51535PurposeExpress His6-tagged HIN domain of mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsHis6 and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-E192C
Plasmid#180233PurposeExpresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistryDepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domains (residue…PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiA
Plasmid#68049PurposeStrain: AM5115, Used for protein purificationDepositorInsertKaiA
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-SPN+IFS
Plasmid#83372PurposeExpresses SPN and IFS complex in E. coli cellDepositorInsertsSPN
IFS
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_ZYMND11_coiled-coil MYND
Plasmid#178493PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertZYMND11_coiled-coil MYND (ZMYND11 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
CNNM3-CBS-YPet
Plasmid#149686PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCNNM3-CBS-YPet (CNNM3 Synthetic, Human)
UseTags8 histidine tagExpressionBacterialMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
C122-E16: CyPet
Plasmid#162896PurposeStandard Gateway entry clone encoding CyPet fluorescent proteinDepositorInsertCyPet fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 A141S
Plasmid#16157DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationA141SPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
PET28-FnCas12a-EP16
Plasmid#183636PurposePET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.DepositorInsertFnCas12a-EP16
UseCRISPRTags6×His TagExpressionBacterialMutationN607R, K613V, N617R, K180S, K660R, D616NPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-His
Plasmid#67866Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tagDepositorInsertMRPP3 (KIAA0391 Human)
UseTagsHisExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-G415R
Plasmid#122693PurposeExpress His-tagged PKM2 (G415R) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationG415RPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Lys13Arg
Plasmid#50726Purposeoverexpression of the human TPI1 Lys13Arg allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Lysine 13 to ArgeninePromoterT7Available sinceFeb. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GST-Q11
Plasmid#185790PurposeEncodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertGST-Q11
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only