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Plasmid#165796PurposeFusion grammar bacterial CP7 promoter, assembles with pHSP70B-TACTDepositorInsertCP7 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCS-TCCC
Plasmid#165774PurposeMultiple cloning site part for enhancer cloning, requires minimal promoter elementDepositorInsertMCS-TCCC
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdSCP
Plasmid#165776PurposeMinimal synthetic Drosophila core promoter, requires enhancer element for full expressionDepositorInsertdSCP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-NT-Tagging
Plasmid#165870PurposeTemplate for amplification of mCherry N-terminal tagging cassetteDepositorInsertVector - NT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mem-TurboID
Plasmid#149409PurposeMammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selectionDepositorInsertmem-TurboID
UseTagsHA and V5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLC-237
Plasmid#73191PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.DepositorInsertParaB-relE
UseTagsExpressionBacterialMutationPromoterParaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLC-246
Plasmid#73190PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-tse2
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
NsvBa
Plasmid#100015PurposeElectrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFPDepositorInsertNsvBa
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5 HA3-eDHFR-T2A-neo
Plasmid#86396Purposefor C-terminal tagging with eDHFR trimethoprim regulated degron with neo selectionDepositorInsertHA3 eDHFR T2A neo
UseCRISPRTagsHA3 eDHFR T2A neoExpressionMutationR12Y, G67S and Y100I mutations in E coli DHFRPromoterAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Omega1
Plasmid#165864PurposeOmega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha1
Plasmid#165862PurposeAlpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3XP3-WhiteA2
Plasmid#165875PurposeBasic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB siteDepositorInsertEye Marker Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLC-239
Plasmid#73193PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-yhaV (yhaV )
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVD2
Plasmid#165857Purposeuniversal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening methodDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD 1-147
Plasmid#165819Purpose1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertGal4BD 1-147
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAL4AD 768-881
Plasmid#165820Purpose768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertGAL4AD 768-881
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Promoterless TP10
Plasmid#165787PurposeDrosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectorsDepositorInsertPromoterless T10
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMR-WhiteA2
Plasmid#165874PurposeBasic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB siteDepositorInsertEye Marker Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-NFAT1(4-460)-GFP
Plasmid#11107PurposeExpresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
UseTags3x HA and GFPExpressionMammalianMutationContains amino acids 4-460 of NFAT1; L78P compareā¦PromoterCMVAvailable sinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-Silent_Mutation_WT
Plasmid#187986Purposehuman p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationsilent mutation for resistance to sip62 #5PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only