Skip to main content
Addgene
Showing: 1 - 14 of 14 results

  2. EFR WT

    Plasmid
    #102413
    Purpose
    fluorescent tagging. Plant expression of EFR WT
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102413-plasmid-map-sequence-id-197797

    Enlarge

  3. EFR Y877F (17)

    Plasmid
    #102415
    Purpose
    fluorescent tagging. Plant expression of EFR Y877F (17)
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Y877F
    Promoter
    35S
    Available since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    102415-plasmid-map-sequence-id-197842

    Enlarge

  4. EFR Y875F (15)

    Plasmid
    #102414
    Purpose
    fluorescent tagging. Plant expression of EFR Y875F (15)
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Y875F
    Promoter
    35S
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102414-plasmid-map-sequence-id-197801

    Enlarge

  10. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194469-plasmid-map-sequence-id-384873

    Enlarge

  11. pRN3P-NLS-EGFP-deadBtgh-3NLS

    Plasmid
    #194470
    Purpose
    Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Mutation
    Aspartic Acid 242 (in Dennis et al.) changed to A…
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194470-plasmid-map-sequence-id-384877

    Enlarge

  12. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

    Enlarge

  13. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

    Enlarge

  14. p35S_GFP-EVDinter-GUS

    Plasmid
    #167123
    Purpose
    Plant binary expression vector containing the intron and proximal plyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.
    Depositor
    Olivier Voinnet, Arturo Marí-Ordóñez
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
    Use
    Tags
    mGFP on N-term, GUS on C-term
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    167123-plasmid-map-sequence-id-331746

    Enlarge

Showing: 1 - 14 of 14 results