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Mouse DECIPHER Lentiviral shRNA Libraries Mod 1 & 2
(Pooled Libraries #28287, #28288)

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  • Purpose

    DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. They are made to cover the entire annotated mouse gene set.

    Module 1 is intended for the widest possible screen and targets annotated pathway genes.

    Module 2 covers disease-associated genes and drug targets that are not included in Module 1.

  • Vector Backbone

    pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro (Plasmid #28289)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 28287 DECIPHER Lentiviral shRNA Library, Mouse Mod 1: Pathway Targets 1
Pooled Library 28288 DECIPHER Lentiviral shRNA Library, Mouse Mod 2: Disease Targets 1
Available to academic and nonprofits only.

Library Details

  • Species
    Mouse
  • Genes targeted

    Module 1: 4,625 Pathway Targets
    Module 2: 4,520 Disease Targets
  • shRNAs
    27,500

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼120 µL
  • Concentration
    1 µg/µL

Resource Information

Depositor Comments

The DECIPHER vector map contains U6, shRNA, T6, barcode, UbiC, RFP, 2A, Puromycin resistance, and 3' delta LTR. The shRNA template insert contains a 21 base pairs sense-GT sequence, a Stem4-Loop-C7-Stem4 element, and a 21 base pair antisense sequence. The 18 base pair barcode region is immediately preceded by a two base pair module identifier where bases CC correspond to module 2 and bases GG correspond to module 3. The mouse module 1 does not have a module identifier. Annealing site for forward primer FwdU6 is located before the shRNA element. Annealing sites for forward primers FwdHTS, and FwdGex are located after the T6 element and before the barcode element. Annealing sites for reverse primers GexSeqN, RevGex and RevHTS are located after the barcode element.

Figure 1. Construction of DECIPHER Libraries.

DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. Mouse Module 2 can be used for the more comprehensive follow-up screens or simply be done in parallel. There are approximately 10,000 genes that have an Entrez/RefSeq ID and at least one interaction documented in PubMed and cover the majority of disease-associated genes and drug targets. About half of these are included in Module 1 while Module 2 covers the rest.

PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website (Link opens in a new window) for more information.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DECIPHER Lentiviral shRNA Library was a gift from Alex Chenchik & Gus Frangou (Addgene #28287)
    DECIPHER Lentiviral shRNA Library was a gift from Alex Chenchik & Gus Frangou (Addgene #28288)
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