Human DECIPHER Lentiviral shRNA Libraries Module 1 & 2
(Pooled Libraries #28285, #28286)
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Purpose
DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. They are made to cover the entire annotated human gene set.
Module 2 covers disease-associated genes and drug targets that are not included in Module 1.
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Vector Backbone
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Depositing Labs
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Publication
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |||
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Pooled Library | 28285 | DECIPHER Lentiviral shRNA Library, Human Mod 1: Pathway Targets | 1 |
Limited Stock Available, 2 units left |
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Pooled Library | 28286 | DECIPHER Lentiviral shRNA Library, Human Mod 2: Disease Targets | 1 |
Library Details
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SpeciesHuman
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Genes targeted
Human Mod 1: Pathway Targets - 5043
Human Mod 2: Disease Targets - 5412 -
shRNAs27,500
Library Shipping
Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼120 µL
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Concentration1 µg/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit Terms- UBMTA
- Life Technologies Corporation End-User Label License for the use of Cellecta Lentiviral Expression System
- Evrogen IP JSC End User Label License for the use of Cellecta lentiviral shRNA constructs comprising TagRFP-encoded gene
- Cellecta Lentiviral Pooled Plasmid and Packaged shRNA Libraries Limited Use Label License
- Cellecta Lentiviral Pooled Plasmid and Packaged shRNA Libraries Ancillary Agreement
- Agilent Technologies Inc End User Label License for the use of Cellecta shRNA libraries comprising Oligo Pools
Industry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. Human Module 2 can be used for the more comprehensive follow-up screens or simply be done in parallel. There are approximately 10,000 genes that have an Entrez/RefSeq ID and at least one interaction documented in PubMed and cover the majority of disease-associated genes and drug targets. About half of these are included in Module 1 while Module 2 covers the rest.
PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website for more information.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DECIPHER Lentiviral shRNA Library, Human Mod 1: Pathway Targets was a gift from Alex Chenchik & Gus Frangou (Addgene #28285)
DECIPHER Lentiviral shRNA Library, Human Mod 2: Disease Targets was a gift from Alex Chenchik & Gus Frangou (Addgene #28286)