Human DECIPHER Lentiviral shRNA Library Mod 3
(Pooled Library #29589)

• Purpose:

  DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. They are made to cover the entire annotated human gene set.

  This module adds additional cell surface, extracellular, and DNA binding targets.

• Vector Backbone: pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro (Plasmid #28289)

• Depositing Labs: Alex Chenchik
• Publication: DECIPHER libraries (unpublished)

Library Details

• Species: Human
• Genes targeted: 4,922
• shRNAs: 27,500

Library Shipping

Each library is delivered in microcentrifuge tubes on blue ice. A tube's contents will not necessarily be frozen. For best results, minimize freeze/thaw cycles.

• Volume: ∼120 µL
• Concentration: 1 µg/µL

Resource Information

• Protocols:
  • DECIPHER Project Website
  • DECIPHER Data and Pathway Analysis Software
  • DECIPHER Resources (includes link to User Manual)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA
• Life Technologies Corporation End-User Label License for the use of Cellecta Lentiviral Expression System
• Evrogen IP JSC End User Label License for the use of Cellecta lentiviral shRNA constructs comprising TagRFP-encoded gene
• Cellecta Lentiviral Pooled Plasmid and Packaged shRNA Libraries Limited Use Label License
• Cellecta Lentiviral Pooled Plasmid and Packaged shRNA Libraries Ancillary Agreement
• Agilent Technologies Inc End User Label License for the use of Cellecta shRNA libraries comprising Oligo Pools

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids need to be grown in OmniMAX 2 T1R (Life Technologies) or SURE (Agilent) Recombination defective E. coli cells.

DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. This module adds additional cell surface, extracellular, DNA binding targets, and other genes to the well-annotated signal transduction and disease-associated genes primarily targeted with the first two modules of the human shRNA library. Many of the genes targeted in Module 3 are conserved and appear integral to a variety of important biological process but are not as well characterized as the 10,000 genes targeted in the first two modules.

PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website for more information.