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FNL Combinatorial Cloning Platform
(Kit # 1000000211 )

Depositing Lab:   Dominic Esposito

A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

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$640 USD + shipping
Available to academics and nonprofits only.
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Original Publication

Combinatorial assembly of clone libraries using site-specific recombination. Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D. Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. PubMed (Link opens in a new window) Article (Link opens in a new window)

Description

The FNL Combinatorial Cloning Platform includes a variety of promoters, fusion tags (N- and C-terminal, reporters, etc.) and Destination vectors for a variety of hosts (E. coli, insect, mammalian). Multiple combinatorial methods are available to maximize the efficiency of reactions and flexibility of the system. The system also can utilize any standard Gateway attL1-attL2 Entry clones including tens of thousands of ORFeome and cDNA clones already available commercially or at Addgene.

Kit Documentation

Protein Science- Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination (PDF, 682 KB) (Link opens in a new window)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The FNL Combinatorial Cloning Platform was a gift from Dominic Esposito (Addgene kit #1000000211).”

For your Reference section:

Combinatorial assembly of clone libraries using site-specific recombination. Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D. Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. PubMed (Link opens in a new window) Article (Link opens in a new window)

FNL Combinatorial Cloning Platform - #1000000211

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

 Spectinomycin
Kanamycin
Chloramphenicol and Ampicillin
Chloramphenicol and Kanamycin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 C121-E01: rLuc
Spectinomycin
Plate 1 / A / 2 C121-E02: ffLuc2
Spectinomycin
Plate 1 / A / 3 C121-E06: gLuc
Spectinomycin
Plate 1 / A / 4 C121-E12: NanoLuc
Spectinomycin
Plate 1 / A / 5 C122-E10: mCherry
Spectinomycin
Plate 1 / A / 6 C122-E11: eGFP
Spectinomycin
Plate 1 / A / 7 C122-E14: mKate
Spectinomycin
Plate 1 / A / 8 C122-E15: mOrange
Spectinomycin
Plate 1 / A / 9 C122-E16: CyPet
Spectinomycin
Plate 1 / A / 10 C122-E17: mPlum
Spectinomycin
Plate 1 / A / 11 C122-E53: mClover3
Spectinomycin
Plate 1 / A / 12 C122-E54: mRuby3
Spectinomycin
Plate 1 / B / 1 C125-E01: stuffer
Spectinomycin
Plate 1 / B / 2 C231-E02: attB2r-NanoLuc-attB3
Spectinomycin
Plate 1 / B / 3 C232-E05: attB2r-meGFP-attB3
Spectinomycin
Plate 1 / B / 4 C232-E06: attB2r-mCherry-attB3
Spectinomycin
Plate 1 / B / 5 C232-E08: attB2r-mTFP1-attB3
Spectinomycin
Plate 1 / B / 6 C232-E25: attB2r-mVenus-attB3
Spectinomycin
Plate 1 / B / 7 C232-E29: attB2r-mClover3-attB3
Spectinomycin
Plate 1 / B / 8 C232-E30: attB2r-mRuby3-attB3
Spectinomycin
Plate 1 / B / 9 C232-E34: attB2r-iRFP670-attB3
Spectinomycin
Plate 1 / B / 10 C234-E05: attB2r-V5-attB3
Spectinomycin
Plate 1 / B / 11 C234-E06: attB2r-FLAG-attB3
Spectinomycin
Plate 1 / B / 12 C234-E07: attB2r-HA-attB3
Spectinomycin
Plate 1 / C / 1 C234-E08: attB2r-3xFLAG-attB3
Spectinomycin
Plate 1 / C / 2 C234-E24: attB2r-Strep2-attB3
Spectinomycin
Plate 1 / C / 3 C234-E25: attB2r-SNAP-attB3
Spectinomycin
Plate 1 / C / 4 C234-E26: attB2r-Halotag-attB3
Spectinomycin
Plate 1 / C / 5 C238-E02: attB2r-BGHpolyA-attB3
Spectinomycin
Plate 1 / C / 6 C238-E07: attB2r-SV40polyA-attB3
Spectinomycin
Plate 1 / C / 7 C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3
Spectinomycin
Plate 1 / C / 8 C239-E03: attB2r-IRES-ffLuc2-pA-attB3
Spectinomycin
Plate 1 / C / 9 C413-E15: attB4-EF1p>-attB1r
Spectinomycin
Plate 1 / C / 10 C413-E21: attB4-PGKp>-attB1r
Spectinomycin
Plate 1 / C / 11 C413-E22: attB4-polyhedrinp>-attB1r
Spectinomycin
Plate 1 / C / 12 C413-E26: attB4-SV40p>-attB1r
Spectinomycin
Plate 1 / D / 1 C413-E31: attB4-TRE3Gp>-attB1r
Spectinomycin
Plate 1 / D / 2 C413-E33: attB4-UbCp>-attB1r
Spectinomycin
Plate 1 / D / 3 C413-E34: attB4-mCMVp>-attB1r
Spectinomycin
Plate 1 / D / 4 C413-E36: attB4-CMV51p>-attB1r
Spectinomycin
Plate 1 / D / 5 C413-E40: attB4-EFSp>-attB1r
Spectinomycin
Plate 1 / D / 6 C413-E38: attB4-T7p>-attB1r
Spectinomycin
Plate 1 / D / 7 C413-E45: attB4-hr5-ie1p>-attB1r
Spectinomycin
Plate 1 / D / 8 C415-E01: attB4-stuffer-attB1r
Spectinomycin
Plate 1 / D / 9 C417-E01: attB4-polyhedrinp>His6-nostop-attB1r
Spectinomycin
Plate 1 / D / 10 C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r
Spectinomycin
Plate 1 / D / 11 C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r
Spectinomycin
Plate 1 / D / 12 C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r
Spectinomycin
Plate 1 / E / 1 C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r
Spectinomycin
Plate 1 / E / 2 C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r
Spectinomycin
Plate 1 / E / 3 C511-E02: attB5r-NanoLuc-nostop-attB1r
Spectinomycin
Plate 1 / E / 4 C511-E15: attB5r-ffluc2-nostop-attB1r
Spectinomycin
Plate 1 / E / 5 C512-E01: attB5r-meGFP-nostop-attB1r
Spectinomycin
Plate 1 / E / 6 C512-E04: attB5r-mCherry-nostop-attB1r
Spectinomycin
Plate 1 / E / 7 C512-E11: attB5r-mTFP1-nostop-attB1r
Spectinomycin
Plate 1 / E / 8 C512-E15: attB5r-mVenus-nostop-attB1r
Spectinomycin
Plate 1 / E / 9 C512-E22: attB5r-mClover3-nostop-attB1r
Spectinomycin
Plate 1 / E / 10 C512-E23: attB5r-mRuby3-nostop-attB1r
Spectinomycin
Plate 1 / E / 11 C512-E29: attB5r-mKate2-nostop-attB1r
Spectinomycin
Plate 1 / E / 12 C512-E34: attB5r-iRFP670-nostop-attB1r
Spectinomycin
Plate 1 / F / 1 C513-E06: attB5r-pA-polyhedrinp>-attB1r
Spectinomycin
Plate 1 / F / 2 C513-E07: attB5r-<p10p-polyhedrinp>-attB1r
Spectinomycin
Plate 1 / F / 3 C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r
Spectinomycin
Plate 1 / F / 4 C514-E09: attB5r-RU5u-FLAG-nostop-attB1r
Spectinomycin
Plate 1 / F / 5 C514-E10: attB5r-RU5u-HA-nostop-attB1r
Spectinomycin
Plate 1 / F / 6 C514-E11: attB5r-RU5u-His6-nostop-attB1r
Spectinomycin
Plate 1 / F / 7 C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r
Spectinomycin
Plate 1 / F / 8 C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r
Spectinomycin
Plate 1 / F / 9 C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r
Spectinomycin
Plate 1 / F / 10 C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r
Spectinomycin
Plate 1 / F / 11 C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r
Spectinomycin
Plate 1 / F / 12 C514-E24: attB5r-RU5u-V5-nostop-attB1r
Spectinomycin
Plate 1 / G / 1 C514-E26: attB5r-RU5u-myc-nostop-attB1r
Spectinomycin
Plate 1 / G / 2 C514-E42: attB5r-RU5u-Strep2-nostop-attB1r
Spectinomycin
Plate 1 / G / 3 C514-E44: attB5r-SNAP-nostop-attB1r
Spectinomycin
Plate 1 / G / 4 C514-E47: attB5r-Halotag-nostop-attB1r
Spectinomycin
Plate 1 / G / 5 C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r
Spectinomycin
Plate 1 / G / 6 C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r
Spectinomycin
Plate 1 / G / 7 C514-E59: attB5r-RU5u-2xHA-nostop-attB1r
Spectinomycin
Plate 1 / G / 8 C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r
Spectinomycin
Plate 1 / G / 9 C515-E01: attB5r-stuffer-attB1r
Spectinomycin
Plate 1 / G / 10 C413-E10: attB4-FerHp>-attB1r
Kanamycin
Plate 1 / G / 11 C413-E11: attB4-Grp78p>-attB1r
Kanamycin
Plate 1 / G / 12 C413-E19: attB4-mPol2p>-attB1r
Kanamycin
Plate 1 / H / 1 C453-E02: attB4-CAGp>-attB5
Kanamycin
Plate 1 / H / 2 C453-E04: attB4-CMV51p>-attB5
Kanamycin
Plate 1 / H / 3 C453-E09: attB4-Grp78p>-attB5
Kanamycin
Plate 1 / H / 4 C453-E14: attB4-EF1p>-attB5
Kanamycin
Plate 1 / H / 5 C453-E17: attB4-PGKp>-attB5
Kanamycin
Plate 1 / H / 6 C453-E18: attB4-polyhedrinp>-attB5
Kanamycin
Plate 1 / H / 7 C453-E21: attB4-FerHp>-attB5
Kanamycin
Plate 1 / H / 8 C453-E23: attB4-TRE3Gp>-attB5
Kanamycin
Plate 1 / H / 9 C453-E24: attB4-UbCp>-attB5
Kanamycin
Plate 1 / H / 10 C453-E25: attB4-mPol2p>-attB5
Kanamycin
Plate 1 / H / 11 C453-E26: attB4-T7p>-attB5
Kanamycin
Plate 1 / H / 12 C455-E01: attB4-stuffer-attB5
Kanamycin
Plate 2 / A / 1 pDest-623 (attR4-attR3)
Chloramphenicol and Ampicillin
Plate 2 / A / 2 pDest-624 (attR4-attR2)
Chloramphenicol and Ampicillin
Plate 2 / A / 3 pDest-639 (attR4-attR2)
Chloramphenicol and Ampicillin
Plate 2 / A / 4 pDest-658 (attR4-attR2)
Chloramphenicol and Ampicillin
Plate 2 / A / 5 pDest-659 (attR4-attR3)
Chloramphenicol and Ampicillin
Plate 2 / A / 6 pDest-663 (attR4-attR2)
Chloramphenicol and Ampicillin
Plate 2 / A / 7 pDest-665 (attR4-attR2)
Chloramphenicol and Ampicillin
Plate 2 / A / 8 pDest-667 (attR4-attR3)
Chloramphenicol and Ampicillin
Plate 2 / A / 9 pDest-669 (attR4-attR3)
Chloramphenicol and Ampicillin
Plate 2 / A / 10 pDest-676 (attR4-attR3)
Chloramphenicol and Ampicillin
Plate 2 / A / 11 pDest-686 (attR4-attR2)
Chloramphenicol and Ampicillin
Plate 2 / A / 12 pDest-894 (attR4-attR2)
Chloramphenicol and Kanamycin
Plate 2 / B / 1 pDest-302 (attR4-attR2)
Chloramphenicol and Ampicillin
Plate 2 / B / 2 pDest-305 (attR4-attR2)
Chloramphenicol and Ampicillin
Plate 2 / B / 3 pDest-311 (attR4-attR3)
Chloramphenicol and Ampicillin
Plate 2 / B / 4 pDest-312 (attR4-attR3)
Chloramphenicol and Ampicillin
Plate 2 / B / 5 pDest-313 (attR4-attR3)
Chloramphenicol and Ampicillin
Data calculated @ 2024-12-31

Kit Plate Map - #1000000211

Plate #2

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