C453-E17: attB4-PGKp>-attB5
(Plasmid
#162976)
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PurposeGateway attB4/attB5 entry clone for mammalian expression using the human PGK promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonr215
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman pGK promoter
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C453-E17: attB4-PGKp>-attB5 was a gift from Dominic Esposito (Addgene plasmid # 162976 ; http://n2t.net/addgene:162976 ; RRID:Addgene_162976) -
For your References section:
Combinatorial assembly of clone libraries using site-specific recombination. Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D. Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. 10.1007/978-1-62703-764-8_14 PubMed 24395366