Fungal Toolkit for Modular Cloning (FTK)
(Kit #
1000000191
)
Depositing Lab: Arnold Driessen
The Fungal Toolkit for Modular Cloning consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native fungal promoters, terminators, selection markers, various CDSs for transcriptional activation and DNA-binding domains, fluorescent reporters, and the AMA1 sequence for fungal autonomous replication as well as CRISPR components such as Cas9, dCas9 sequences, and single guide RNA (sgRNA) transcription units for filamentous fungi.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publications
Modular Synthetic Biology Toolkit for Filamentous Fungi Mózsik L, Pohl C, Meyer V, Bovenberg RAL, Nygård Y, Driessen AJM ACS Synthetic Biology. 2021 Nov 19; doi: 10.1021/acssynbio.1c00260. PubMed PMID: 34726388 .
CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mózsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygård Y, Driessen AJM Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. PubMed PMID: 34726388 .
Description
The Fungal Toolkit for Modular Cloning consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native fungal promoters, terminators, selection markers, various CDSs for transcriptional activation and DNA-binding domains, fluorescent reporters, and the AMA1 sequence for fungal autonomous replication as well as CRISPR components such as Cas9, dCas9 sequences, and single guide RNA (sgRNA) transcription units for filamentous fungi.
List of vectors in the Fungal Toolkit for Modular Cloning (FTK).
(a) Location of genetic parts in a transcription unit with their corresponding linker sequences.
(b) List of parts of the toolkit, containing promoters (P1), UASs (P2), UAS-compatible core promoters (P3), coding sequences with various fusion possibilities (CDS1–5), terminators (T1), complete transcription units (TUs), and additional vectors (sgRNA transcription units, flanking sequences, and AMA1 vectors).
Abbreviations (Pc, An, Ania, Ao, Sc) indicate the origin of the template (P. rubens, A. niger, A. nidulans, A. oryzae, S. cerevisiae, respectively).
(Image from Mózsik et al. ACS Synth Biol (2021) 10:11 https://doi.org/10.1021/acssynbio.1c00260, reprinted under the terms of the Creative Commons Attribution License.)
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Fungal Toolkit for Modular Cloning (FTK) was a gift from Arnold Driessen (Addgene kit #1000000191).”
For your Reference section:
Modular Synthetic Biology Toolkit for Filamentous Fungi Mózsik L, Pohl C, Meyer V, Bovenberg RAL, Nygård Y, Driessen AJM ACS Synthetic Biology. 2021 Nov 19; doi: 10.1021/acssynbio.1c00260. PubMed PMID: 34726388 .
CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mózsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygård Y, Driessen AJM Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. PubMed PMID: 34726388 .
Fungal Toolkit for Modular Cloning (FTK) - #1000000191
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Spectinomycin | |
| Ampicillin | |
| Chloramphenicol |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pFTK001 | Spectinomycin |
| A / 2 | pFTK002 | Spectinomycin |
| A / 3 | pFTK003 | Spectinomycin |
| A / 4 | pFTK004 | Spectinomycin |
| A / 5 | pFTK005 | Spectinomycin |
| A / 6 | pFTK006 | Spectinomycin |
| A / 7 | pFTK007 | Spectinomycin |
| A / 8 | pFTK008 | Spectinomycin |
| A / 9 | pFTK009 | Spectinomycin |
| A / 10 | pFTK010 | Spectinomycin |
| A / 11 | pFTK011 | Spectinomycin |
| A / 12 | pFTK012 | Spectinomycin |
| B / 1 | pFTK013 | Spectinomycin |
| B / 2 | pFTK014 | Spectinomycin |
| B / 3 | pFTK015 | Spectinomycin |
| B / 4 | pFTK016 | Spectinomycin |
| B / 5 | pFTK017 | Spectinomycin |
| B / 6 | pFTK018 | Spectinomycin |
| B / 7 | pFTK019 | Spectinomycin |
| B / 8 | pFTK020 | Spectinomycin |
| B / 9 | pFTK021 | Spectinomycin |
| B / 10 | pFTK022 | Spectinomycin |
| B / 11 | pFTK023 | Spectinomycin |
| B / 12 | pFTK024 | Spectinomycin |
| C / 1 | pFTK025 | Spectinomycin |
| C / 2 | pFTK026 | Spectinomycin |
| C / 3 | pFTK027 | Spectinomycin |
| C / 4 | pFTK028 | Spectinomycin |
| C / 5 | pFTK029 | Spectinomycin |
| C / 6 | pFTK030 | Spectinomycin |
| C / 7 | pFTK031 | Spectinomycin |
| C / 8 | pFTK032 | Spectinomycin |
| C / 9 | pFTK033 | Spectinomycin |
| C / 10 | pFTK034 | Spectinomycin |
| C / 11 | pFTK035 | Spectinomycin |
| C / 12 | pFTK036 | Spectinomycin |
| D / 1 | pFTK037 | Spectinomycin |
| D / 2 | pFTK038 | Spectinomycin |
| D / 3 | pFTK039 | Spectinomycin |
| D / 4 | pFTK040 | Spectinomycin |
| D / 5 | pFTK041 | Spectinomycin |
| D / 6 | pFTK042 | Spectinomycin |
| D / 7 | pFTK043 | Spectinomycin |
| D / 8 | pFTK044 | Spectinomycin |
| D / 9 | pFTK045 | Spectinomycin |
| D / 10 | pFTK046 | Spectinomycin |
| D / 11 | pFTK047 | Spectinomycin |
| D / 12 | pFTK048 | Spectinomycin |
| E / 1 | pFTK049 | Spectinomycin |
| E / 2 | pFTK050 | Spectinomycin |
| E / 3 | pFTK051 | Spectinomycin |
| E / 4 | pFTK052 | Spectinomycin |
| E / 5 | pFTK053 | Spectinomycin |
| E / 6 | pFTK054 | Spectinomycin |
| E / 7 | pFTK055 | Spectinomycin |
| E / 8 | pFTK056 | Spectinomycin |
| E / 9 | pFTK057 | Spectinomycin |
| E / 10 | pFTK058 | Spectinomycin |
| E / 11 | pFTK059 | Spectinomycin |
| E / 12 | pFTK060 | Spectinomycin |
| F / 1 | pFTK061 | Spectinomycin |
| F / 2 | pFTK062 | Spectinomycin |
| F / 3 | pFTK063 | Spectinomycin |
| F / 4 | pFTK064 | Spectinomycin |
| F / 5 | pFTK065 | Spectinomycin |
| F / 6 | pFTK066 | Spectinomycin |
| F / 7 | pFTK067 | Spectinomycin |
| F / 8 | pFTK068 | Spectinomycin |
| F / 9 | pFTK069 | Spectinomycin |
| F / 10 | pFTK070 | Spectinomycin |
| F / 11 | pFTK071 | Spectinomycin |
| F / 12 | pFTK072 | Spectinomycin |
| G / 1 | pFTK073 | Spectinomycin |
| G / 2 | pFTK074 | Spectinomycin |
| G / 3 | pFTK075 | Spectinomycin |
| G / 4 | pFTK076 | Spectinomycin |
| G / 5 | pFTK077 | Spectinomycin |
| G / 6 | pFTK078 | Spectinomycin |
| G / 7 | pFTK079 | Spectinomycin |
| G / 8 | pFTK080 | Spectinomycin |
| G / 9 | pFTK081 | Spectinomycin |
| G / 10 | pFTK082 | Spectinomycin |
| G / 11 | pFTK083 | Spectinomycin |
| G / 12 | pFTK084 | Spectinomycin |
| H / 1 | pFTK085 | Spectinomycin |
| H / 2 | pFTK086 | Spectinomycin |
| H / 3 | pFTK087 | Spectinomycin |
| H / 4 | pFTK088 | Spectinomycin |
| H / 5 | pFTK089 | Spectinomycin |
| H / 6 | pFTK090 | Spectinomycin |
| H / 7 | pFTK091 | Ampicillin |
| H / 8 | pFTK092 | Ampicillin |
| H / 9 | pFTK093 | Ampicillin |
| H / 10 | pFTK094-LM-AMA002.0 | Chloramphenicol |
| H / 11 | pLM-AMA15.0 Cas9 | Chloramphenicol |
| H / 12 | pLM-AMA18.0 dCas9-VPR | Chloramphenicol |