Structure of the nutrient-sensing hub GATOR2.
Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, Chantranupong L, Kolibius J, Quast JP, Sabatini DM
Nature. 2022 Jul;607(7919):610-616. doi: 10.1038/s41586-022-04939-z. Epub 2022 Jul 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184561 | Flag-MIOS(1-875) in pRK5 | CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells. |
| 184562 | K-to-R mutant NPRL2(1-380) in pLJM60 | CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines. |
| 184563 | K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized | CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines. |