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Dynamical localization of a thylakoid membrane binding protein is required for acquisition of photosynthetic competency.
Gutu A, Chang F, O'Shea EK
Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912. PubMed Article

Plasmids from Article

ID Plasmid Purpose
107249pAGH42Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).
107254pAGH50Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.
107255pAGH51HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.
107262pAGH23contains CurT-mTurquoise2 construct used to replace the native CurT in Synechocystis PCC 6803 via homologous recombination.

Antibodies from Article