PI(3,4,5)P3 Engagement Restricts Akt Activity to Cellular Membranes.
Ebner M, Lucic I, Leonard TA, Yudushkin I
Mol Cell. 2017 Feb 2;65(3):416-431.e6. doi: 10.1016/j.molcel.2016.12.028.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86561 | pFBD-Akt1(WT) | Expresses human full-length Akt1(WT) |
| 86562 | pFBD-Akt1(K14A) | Expresses human full-length Akt1(K14A) mutant |
| 86563 | pFBD-Akt1(E17K) | Expresses human full-length Akt1(E17K) mutant |
| 86576 | pFBD-Akt13C(WT) | Expresses cleavable human full-length Akt1(WT) |
| 86577 | pFBD-Akt13C(S473D) | Expresses cleavable human full-length Akt1(S473D) phosphomimetic |
| 86578 | pFBD-Akt1(T308A/S473A) | Expresses human full-length Akt1(T308A/S473A) phosphomutant |
| 86591 | pFBD-Btk(PH)Akt1(KD) | Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1 |
| 86592 | pFBD-Akt1(D323A/D325A) | Expresses human full-length Akt1(D323A/D325A) mutant |
| 86593 | pEGFP-Akt2 | Expresses eGFP-tagged human Akt2(WT) |
| 86594 | pTagBFP-Akt1 | Expresses TagBFP-tagged human Akt1(WT) |
| 86595 | pEGFP-Akt2(K14A/R25E) | Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant |
| 86596 | pmCherry-PH(Akt2) | Expresses mCherry-tagged PH(WT) domain of human Akt2 |
| 86597 | pFKBPmCherry-Akt2(K14A) | Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant |
| 86598 | pFKBPmCherry-Akt2(KD) | Expresses recruitable mCherry-tagged kinase domain of human Akt2(WT) |
| 86599 | pEGFP-PH1(WT) | Expresses eGFP-tagged PH(WT) domain of human Akt1 |
| 86600 | pmCherry-PH1(K14A) | Expresses mCherry-tagged PH(K14A) domain of human Akt1 |
| 86623 | pmCherry-Akt2(WT) | Expresses mCherry-tagged human Akt2(WT) |
| 86624 | pmCherry-Akt2(K14A) | Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant |
| 86625 | pEGFP-Akt2(T309A) | Expresses eGFP-tagged human Akt2(T309A) phosphomutant |
| 86626 | pHaloTag-PS | Expresses Akt pseudosubstrate tagged with a HaloTag |
| 86627 | pHaloTag-PS(RA) | Expresses Akt pseudosubstrate (P-3 RA) mutant tagged with a HaloTag |
| 86628 | pmCherry-PS | Expresses Akt pseudosubstrate tagged with mCherry |
| 86629 | pHaloTag-EGFP | Expresses HaloTag-eGFP tandem (cytosolic) |
| 86630 | pEGFP-Akt1(D323A/D325A) | Expresses eGFP-tagged human Akt1(D323A/D325A) mutant |
| 86631 | pmCherry-Akt1(WT) | Expresses mCherry-tagged human Akt1(WT) |
| 86632 | pEGFP-Akt2(K14A/R25E)-Halo-PS | Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem) |
| 86633 | pHR-FKBP:mCherry-KRas4Bc30 | Expresses mCherry-tagged membrane recruiter construct |
| 86634 | pHR-FRB:Akt1(D274A) | Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant |
| 86635 | pEGFP-PH(Btk) | Expresses eGFP-taggd PH domain of Btk |
| 86636 | pmCherry-PH(Btk)KD(Akt1) | Expresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1 |
| 86637 | pEGFP-Akt1(WT) | Expresses eGFP-tagged human Akt1(WT) |
| 86638 | pHR-FKBP:mCherry-Rab7a | Expresses mCherry-tagged Rab7a |
| 86639 | pmCherry-EGFP | Expresses mCherry-eGFP tandem (cytoplasmic) |