Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120.

Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP
J Bacteriol. 1997 Mar;179(6):1998-2005. (Link opens in a new window) PubMed

Plasmids from Article

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ID	Plasmid	Purpose
70690	pRL1124	provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.
70282	pRL542	ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes
70261	pRL443	Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.
58494	pRL623	helper plasmid for bacterial conjugal DNA transfer