GeneGuard: a Modular Plasmid System Designed for Biosafety.
Wright O, Delmans M, Stan GB, Ellis T
ACS Synth Biol. 2014 May 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58296 | pSEVAT1Z1A | GeneGuard vector cassette: thyA, R6K COR, Zeta toxin; with ampR as cargo. |
| 58297 | pSEVAD1Z1A | GeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as cargo. |
| 58298 | pSEVAT1K1A | GeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as cargo. |
| 58299 | pSEVAD1K1A | GeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as cargo. |
| 58300 | pSEVAT7Z1A | GeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo. |
| 58301 | pSEVAD7Z1A | GeneGuard vector cassette: dapA, ColE2 COR, Zeta toxin; with ampR as cargo. |
| 58302 | pSEVAT7K1A | GeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo. |
| 58303 | pSEVAD7K1A | GeneGuard vector cassette: dapA, ColE2 COR, Kid toxin; with ampR as cargo. |
| 58304 | pSEVA12DKR-A | GeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K. |
| 58305 | pSEVA12DKR-E | GeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K. |
| 58306 | pSEVA12DKR-J | GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K. |
| 58307 | pSEVA12TEC-B | GeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z. |
| 58308 | pSEVA12TEC-I | GeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z. |
| 58310 | pSEVA3b61 | Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner. |
| 58311 | pSEVA3b67Rb | Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info. |