Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA.
Abil Z, Denard CA, Zhao H
J Biol Eng. 2014 Mar 1;8(1):7.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52136 | pChlr-pNEB193 | A backbone plasmid for creation of additional PUF repeat modules |
| 52137 | pET28-GG-PUF | Receiving vector for PUM-HD assembly and expression in E.coli |
| 52138 | pCMV-TTP(C147R)-GG-PUF | Receiving vector for PUM-HD assembly and expression in mammalian cells |
| 52139 | pChlr-1SQ | Encodes module 1 with amino acids 12S-16Q for recognition of nt 8A |
| 52140 | pChlr-1SE | Encodes module 1 with amino acids 12S-16E for recognition of nt 8G |
| 52141 | pChlr-1NQ | Encodes module 1 with amino acids 12N-16Q for recognition of nt 8U |
| 52142 | pChlr-1SR | Encodes module 1 with amino acids 12S-16R for recognition of nt 8C |
| 52143 | pChlr-2CQ | Encodes module 2 with amino acids 12C-16Q for recognition of nt 7A |
| 52144 | pChlr-2SE | Encodes module 2 with amino acids 12S-16E for recognition of nt 7G |
| 52145 | pChlr-2NQ | Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U |
| 52146 | pChlr-2SR | Encodes module 2 with amino acids 12S-16R for recognition of nt 7C |
| 52147 | pChlr-3CQ | Encodes module 3 with amino acids 12C-16Q for recognition of nt 6A |
| 52148 | pChlr-3SE | Encodes module 3 with amino acids 12S-16E for recognition of nt 6G |
| 52149 | pChlr-3NQ | Encodes module 3 with amino acids 12N-16Q for recognition of nt 6U |
| 52150 | pChlr-3SR | Encodes module 3 with amino acids 12S-16R for recognition of nt 6C |
| 52151 | pChlr-4CQ | Encodes module 4 with amino acids 12C-16Q for recognition of nt 5A |
| 52152 | pChlr-4SE | Encodes module 4 with amino acids 12S-16E for recognition of nt 5G |
| 52153 | pChlr-4NQ | Encodes module 4 with amino acids 12N-16Q for recognition of nt 5U |
| 52154 | pChlr-4SR | Encodes module 4 with amino acids 12S-16R for recognition of nt 5C |
| 52155 | pChlr-4CYQ | Encodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5A |
| 52156 | pChlr-4SYE | Encodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G |
| 52157 | pChlr-4NYQ | Encodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5U |
| 52158 | pChlr-4SYR | Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C |
| 52159 | pChlr-5CQ | Encodes module 5 with amino acids 12C-16Q for recognition of nt 4A |
| 52160 | pChlr-5SE | Encodes module 5 with amino acids 12S-16E for recognition of nt 4G |
| 52161 | pChlr-5NQ | Encodes module 5 with amino acids 12N-16Q for recognition of nt 4U |
| 52162 | pChlr-5SR | Encodes module 5 with amino acids 12S-16R for recognition of nt 4C |
| 52163 | pChlr-6CQ | Encodes module 6 with amino acids 12C-16Q for recognition of nt 3A |
| 52164 | pChlr-6SE | Encodes module 6 with amino acids 12S-16E for recognition of nt 3G |
| 52165 | pChlr-6NQ | Encodes module 6 with amino acids 12N-16Q for recognition of nt 3U |
| 52166 | pChlr-6SR | Encodes module 6 with amino acids 12S-16R for recognition of nt 3C |
| 52167 | pChlr-7CQ | Encodes module 7 with amino acids 12C-16Q for recognition of nt 2A |
| 52168 | pChlr-7SE | Encodes module 7 with amino acids 12S-16E for recognition of nt 2G |
| 52169 | pChlr-7NQ | Encodes module 7 with amino acids 12N-16Q for recognition of nt 2U |
| 52170 | pChlr-7SYR | Encodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2C |
| 52171 | pChlr-8CQ | Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A |
| 52172 | pChlr-8SE | Encodes module 8 with amino acids 12S-16E for recognition of nt 1G |
| 52173 | pChlr-8NQ | Encodes module 8 with amino acids 12N-16Q for recognition of nt 1U |
| 52174 | pChlr-8SR | Encodes module 8 with amino acids 12S-16R for recognition of nt 1C |