DNA Replicons for Plant Genome Engineering.
Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51491 | pREP | pMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virus |
| 51492 | pREPK219H | pREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication. |
| 51493 | pLSL | T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence. |
| 51494 | pLSLGFP | pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid |
| 51495 | pLSLZ | pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid |
| 51496 | pLSLZ.D | pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid |
| 51498 | pLSLT | pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid |
| 51500 | pLSLR | Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation. |
| 51501 | pLSLGFP.R | Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon |
| 51510 | pLSLZ.D.R | Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR. |
| 51511 | pZLSLD.R | Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template. |
| 51512 | p35SZ.D | pMDC32 with Zif268:FokI followed by us:NPTII repair template sequence |
| 51513 | p35SZ | pMDC32 with Zif268:FokI followed by approximately 3kb of filler sequence |
| 51514 | p35ST | pMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule |
| 51517 | pLSLGUS | pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid |
| 51518 | pNJB80 | Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently. |
| 51519 | pNJB91 | Gateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI. |
| 51520 | pNJB98 | Gateway entry vector with attL5 and attL2 sites flanking us:NPTII repair template sequence |
| 51521 | pNJB99 | Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence |