Discovery of FERM domain protein-protein interaction inhibitors for MSN and CD44 as a potential therapeutic approach for Alzheimer's disease.
Du Y, Bradshaw WJ, Leisner TM, Annor-Gyamfi JK, Qian K, Bashore FM, Sikdar A, Nwogbo FO, Ivanov AA, Frye SV, Gileadi O, Brennan PE, Levey AI, Axtman AD, Pearce KH, Fu H, Katis VL
J Biol Chem. 2023 Oct 20:105382. doi: 10.1016/j.jbc.2023.105382.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175466 | MSNA-c001 | Protein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ). |
| 175468 | MSNA-c021 | Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44. |
| 175470 | MSNA-c028 | Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44. |
| 175471 | MSNA-c029 | Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44. |
| 211823 | pDEST27-GST-hMSN | Express human MSN in mammalian cells |
| 211824 | pFUW-VenusFlag-hCD44 | Express CD44 in mammalian cells |