Selenoprotein S Interacts with the Replication and Transcription Complex of SARS-CoV-2 by Binding nsp7.
Ghelichkhani F, Gonzalez FA, Kapitonova MA, Rozovsky S
J Mol Biol. 2023 Apr 15;435(8):168008. doi: 10.1016/j.jmb.2023.168008. Epub 2023 Feb 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201022 | pETDuet-1-6xHis-TEV-Nsp8 | Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site. |
| 201023 | pET-28a(+)-6xHis-TEV-ORF9c | Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site. |
| 201024 | pET-Duet-6xHis-TEV-Nsp8(76-198) | Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site. |
| 201025 | pcDNA5-FRT-TO-nsp7 | Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian. |
| 201026 | pcDNA 3.1-nsp7 | Expresses SARS-CoV-2 nsp7 in mammalian. |
| 201027 | pcDNA 3.1-nsp7-FLAG | Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian. |