pcDNA 3.1-nsp7
(Plasmid
#201026)
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PurposeExpresses SARS-CoV-2 nsp7 in mammalian.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 5636
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Modifications to backboneInsertion of nsp7
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenon-structural protein 7
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Alt namensp7
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SpeciesSynthetic; Severe acute respiratory syndrome coronavirus 2
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Insert Size (bp)255
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter T7 promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA 3.1-nsp7 was a gift from Sharon Rozovsky (Addgene plasmid # 201026 ; http://n2t.net/addgene:201026 ; RRID:Addgene_201026) -
For your References section:
Selenoprotein S Interacts with the Replication and Transcription Complex of SARS-CoV-2 by Binding nsp7. Ghelichkhani F, Gonzalez FA, Kapitonova MA, Rozovsky S. J Mol Biol. 2023 Apr 15;435(8):168008. doi: 10.1016/j.jmb.2023.168008. Epub 2023 Feb 10. 10.1016/j.jmb.2023.168008 PubMed 36773692
Map uploaded by the depositor.
