Genetically manipulating endogenous Kras levels and oncogenic mutations in vivo influences tissue patterning of murine tumorigenesis.
Le Roux O, Pershing NLK, Kaltenbrun E, Newman NJ, Everitt JI, Baldelli E, Pierobon M, Petricoin EF, Counter CM
Elife. 2022 Sep 7;11:e75715. doi: 10.7554/eLife.75715.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206842 | pcDNA3.1+FLAG-KrasnatG12D | To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag. |
| 206843 | pcDNA3.1+FLAG-KrasnatQ61R | To express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag. |
| 206844 | pcDNA3.1+FLAG-KrascomG12D | To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag. |
| 206845 | pcDNA3.1+FLAG-KrascomQ61R | To express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag. |
| 206862 | pBabePuro-FLAG-KrascomG12D | To stably express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag in cells resistant to Neo.. |
| 206863 | pBabePuro-FLAG-KrascomQ61R | To stably express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag in cells resistant to Neo. |
| 206864 | pBabePuro-FLAG-KrasnatQ61R | To stably express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag in cells resistant to Neo. |
| 206865 | pBabePuro-FLAG-KrasnatG12D | To stably express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag in cells resistant to Neo. |