Skip to main content
Addgene

Canonical BAF complex regulates the oncogenic program in human T-cell acute lymphoblastic leukemia.

Aoki K, Hyuga M, Tarumoto Y, Nishibuchi G, Ueda A, Ochi Y, Sugino S, Mikami T, Kobushi H, Kato I, Akahane K, Inukai T, Takaori-Kondo A, Takita J, Ogawa S, Yusa K
Blood. 2023 Nov 3:blood.2023020857. doi: 10.1182/blood.2023020857. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
200100pKLV2-EF1a-Cas9GFP-WLentiviral vector expressing Cas9 fused with GFP at the C-terminus
200101pKLV-EF1a-mCherryLuciferase-WLentiviral vector expressing mCherry fused with Luciferase at the C-terminus
200102pKLV-EF1a-mCherryCXCR4-WLentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus
200459pKLV2-U6gRNA5(AAVS1)-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human AAVS1
200460pKLV2-U6gRNA5(CXCR4(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4
200461pKLV2-U6gRNA5(CXCR4(16))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4
200462pKLV2-U6gRNA5(SMARCA4(15))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human SMARCA4
200464pKLV2-U6gRNA5(SMARCA4(43))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human SMARCA4
200465pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human SMARCA2
200467pKLV2-U6gRNA5(ARID1A(42))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human ARID1A
200468pKLV2-U6gRNA5(ARID1A(44))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human ARID1A
200469pKLV2-U6gRNA5(ARID1B(21))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human ARID1B
200470pKLV2-U6gRNA5(ARID1B(23))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human ARID1B
200471pKLV2-U6gRNA5(ARID2(13))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human ARID2
200472pKLV2-U6gRNA5(ARID2(14))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human ARID2
200473pKLV2-U6gRNA5(BRD9(13))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BRD9
200474pKLV2-U6gRNA5(BRD9(91))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BRD9
200475pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting GFP
200476pKLV2-U6gRNA5(CXCR4-SE(95))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-SE
200477pKLV2-U6gRNA5(CXCR4-SE(96))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-SE
200478pKLV2-U6gRNA5(CXCR4-E1(15))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-E1
200479pKLV2-U6gRNA5(CXCR4-E1(16))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-E1
200480pKLV2-U6gRNA5(CXCR4-E2(37))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-E2
200481pKLV2-U6gRNA5(CXCR4-E2(38))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-E2
200482pKLV2-U6gRNA5(CXCR4-E3(45))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-E3
200483pKLV2-U6gRNA5(CXCR4-E3(48))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-E3
200484pKLV2-U6gRNA5(CXCR4-E4(56))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-E4
200485pKLV2-U6gRNA5(CXCR4-E4(59))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CXCR4-E4
200486pKLV2-U6gRNA5(CDK6-E1(1))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CDK6-E1
200487pKLV2-U6gRNA5(CDK6-E1(6))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CDK6-E1
200488pKLV2-U6gRNA5(CDK6-E2(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CDK6-E2
200489pKLV2-U6gRNA5(CDK6-E2(15))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human CDK6-E2
200490pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human RIC8A
200491pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human RIC8A
200492pKLV2-U6gRNA5(RHOA(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human RHOA
200493pKLV2-U6gRNA5(RHOA(15))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human RHOA
200494pKLV2-U6gRNA5(NDUFA1(11))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human NDUFA1
200495pKLV2-U6gRNA5(NDUFA1(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human NDUFA1
200496pKLV2-U6gRNA5(MRPL21(14))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human MRPL21
200497pKLV2-U6gRNA5(MRPL21(16))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human MRPL21
200498pKLV2-U6gRNA5(MRPS26(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human MRPS26
200499pKLV2-U6gRNA5(MRPS26(15))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human MRPS26
200500pKLV2-U6gRNA5(RUNX1(11))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human RUNX1
200501pKLV2-U6gRNA5(RUNX1(13))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human RUNX1
200502pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human AAVS1
200503pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1
200504pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2
200505pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2
200506pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(AAVS1)-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human ARID1A and AAVS1
200507pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(21))-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human ARID1A and ARID1B
200508pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(23))-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human ARID1A and ARID1B
200510pKLV2-U6gRNA5(SMARCA2(47))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human SMARCA2
208545pKLV2-U6gRNA5(TAL1(11))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human TAL1
208546pKLV2-U6gRNA5(TAL1(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human TAL1
208547pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human GATA3
208548pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human GATA3
208549pKLV2-U6gRNA5(ETS1(11))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human ETS1
208550pKLV2-U6gRNA5(ETS1(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human ETS1
208551pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human RUNX3
208552pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human RUNX3
208554pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BCL11B-E1
208555pKLV2-U6gRNA5(BCL11B-E2(24))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BCL11B-E2
208556pKLV2-U6gRNA5(BCL11B-E2(25))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BCL11B-E2
208557pKLV2-U6gRNA5(BCL11B-E3(32))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BCL11B-E3
208558pKLV2-U6gRNA5(BCL11B-E3(34))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BCL11B-E3
208559pKLV2-U6gRNA5(BCL11B-E4(42))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BCL11B-E4
208560pKLV2-U6gRNA5(BCL11B-E4(44))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BCL11B-E4
208569pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(12))-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human RUNX1 and RUNX3
208570pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(13))-PGKpuroBFP-WLentiviral vector expressing gRNA targeting human RUNX1 and RUNX3
208756pKLV-EF1a-BFPCDK6-WLentiviral vector expressing BFP fused with CDK6 cDNA
208757pKLV-EF1a-BFP-WLentiviral vector expressing BFP
208758pKLV-EF1a-mCherryBCL11B-WLentiviral vector expressing mCherry fused with BCL11B cDNA
210612pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-WLentiviral vector expressing gRNA targeting a control region at the CXCR4 locus
210613pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-WLentiviral vector expressing gRNA targeting a control region at the CXCR4 locus
210614pKLV2-U6gRNA5(CXCR4-E4(56))-PGKpuro2AmCherry-WLentiviral vector expressing gRNA targeting human CXCR4-E4
210615pKLV2-U6gRNA5(CXCR4-E4(59))-PGKpuro2AmCherry-WLentiviral vector expressing gRNA targeting human CXCR4-E4
212116pKLV2-U6gRNA5(BCL11B-E1(13))-PGKpuro2ABFP-WLentiviral vector expressing gRNA targeting human BCL11B-E1

Antibodies from Article