A DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology.
Campbell BFN, Dittmann A, Dreier B, Pluckthun A, Tyagarajan SK
Elife. 2022 Oct 31;11:e80895. doi: 10.7554/eLife.80895.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199610 | pEGFPC2-gephyrin C3 | Encodes N-terminal eGFP-tagged gephyrin including the C3 splice cassette for expression in mammalian cells. |
| 199611 | pEGFPC2-gephyrin C4a | Encodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells. |
| 199612 | pQIq_MRGS_HIS8_( E3_5)_FLAG | Encodes DARPin-FLAG E3_5 for bacterial expression and column purification |
| 199613 | pQIq_MRGS_HIS8_(27B3)_FLAG | Encodes DARPin-FLAG 27B3 for bacterial expression and column purification |
| 199615 | pQIq_MRGS_HIS8_(27F3)_FLAG | Encodes DARPin-FLAG 27F3 for bacterial expression and column purification |
| 199616 | pQIq_MRGS_HIS8_(27G2)_FLAG | Encodes DARPin-FLAG 27G2 for bacterial expression and column purification |
| 199617 | pcDNA3.1_ E3_5 _hFc | Encodes DARPin-hFc E3_5 for mammalian expression and column purification |
| 199618 | pcDNA3.1_27B3_hFc | Encodes DARPin-hFc 27B3 for mammalian expression and column purification |
| 199619 | pcDNA3.1_27F3_hFc | Encodes DARPin-hFc 27F3 for mammalian expression and column purification |