Nonmonotone invasion landscape by noise-aware control of metastasis activator levels.

Wan Y, Cohen J, Szenk M, Farquhar KS, Coraci D, Krzyszton R, Azukas J, Van Nest N, Smashnov A, Chern YJ, De Martino D, Nguyen LC, Bien H, Bravo-Cordero JJ, Chan CH, Rosner MR, Balazsi G
Nat Chem Biol. 2023 Jul;19(7):887-899. doi: 10.1038/s41589-023-01344-z. Epub 2023 May 25. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
199212	LPUtopia-7	Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker
199213	pCAG-eSpCas9_sgAAVS1	All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.
199214	pCMV-BACH1t_RFP	BACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone.
199215	pCMV-RKIP_RFP	RKIP (PEBP1) expression plasmid with a RFP reporter on the backbone.
199216	pUC_hU6-shRKIP_BFP	Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.
199217	pUC-hU6-shBACH1_BFP	Expression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone.
199218	pUt-mNF-BACH1 Cas9-Resistant	LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection.
199219	pUt-mNF-BACH1	LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection.
199220	pUt-mNF-GFP	LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.
199221	pUt-mPF-BACH1 Cas9-Resistant_AmpR	LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection.
199222	pUt-mPF-GFP	LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.