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Direct interrogation of context-dependent GPCR activity with a universal biosensor platform.

Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
189731pLVX-CMV-Galpha-s ONE-GOGPCR/G protein BRET biosensor
189732pLVX-CMV-Galpha-oA ONE-GOGPCR/G protein BRET biosensor
189733pLVX-CMV-Galpha-oB ONE-GOGPCR/G protein BRET biosensor
189734pLVX-CMV-Galpha-z ONE-GOGPCR/G protein BRET biosensor
189735pLVX-CMV-Galpha-i1 ONE-GOGPCR/G protein BRET biosensor
189736pLVX-CMV-Galpha-i2 ONE-GOGPCR/G protein BRET biosensor
189737pLVX-CMV-Galpha-i3 ONE-GOGPCR/G protein BRET biosensor
189738pLVX-CMV-Galpha-q ONE-GOGPCR/G protein BRET biosensor
189739pLVX-CMV-Galpha-12 ONE-GOGPCR/G protein BRET biosensor
189740pLVX-CMV-Galpha-13 ONE-GOGPCR/G protein BRET biosensor
204324pcDNA3.1-mas-KB1691-NlucGPCR/G protein BRET biosensor
204325pcDNA3.1-mas-KB2123-NlucGPCR/G protein BRET biosensor
204326pcDNA3.1-cyto-KB1691-NlucGPCR/G protein BRET biosensor
204327pLVX-SV40-Galpha-s ONE-GOGPCR/G protein BRET biosensor
204328pLVX-SV40-Galpha-i1 ONE-GOGPCR/G protein BRET biosensor
204329pLVX-SV40-Galpha-i3 ONE-GOGPCR/G protein BRET biosensor
204330pLVX-SV40-Galpha-oA ONE-GOGPCR/G protein BRET biosensor
204331pLVX-SV40-Galpha-q ONE-GOGPCR/G protein BRET biosensor
204332pLVX-SV40-Galpha-13 ONE-GOGPCR/G protein BRET biosensor
204333pLVX-hSyn-Galpha-i1 ONE-GOGPCR/G protein BRET biosensor
204334pLVX-hSyn-Galpha-i3 ONE-GOGPCR/G protein BRET biosensor
204335pLVX-hSyn-Galpha-oA ONE-GOGPCR/G protein BRET biosensor
204336pLVX-hSyn-Galpha-q ONE-GOGPCR/G protein BRET biosensor
204337pLVX-hSyn-Galpha-13 ONE-GOGPCR/G protein BRET biosensor
204338pLVX-CMV-Galpha-i3 G183S ONE-GOGPCR/G protein BRET biosensor
204339pLVX-CMV-IRESWT-Gbetagamma ONE-GOGPCR/G protein BRET biosensor
204340pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GOGPCR/G protein BRET biosensor
204341pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GOGPCR/G protein BRET biosensor
204342pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GOGPCR/G protein BRET biosensor
204343pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GOGPCR/G protein BRET biosensor
214371pLVX-hSyn-Galpha-s ONE-GOGPCR/G protein BRET biosensor

Antibodies from Article