Dissecting protein tyrosine phosphatase signaling by engineered chemogenetic control of its activity.
Fauser J, Huyot V, Matsche J, Szynal BN, Alexeev Y, Kota P, Karginov AV
J Cell Biol. 2022 Aug 1;221(8). pii: 213352. doi: 10.1083/jcb.202111066. Epub 2022 Jul 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188655 | RapR-Shp2-mVenus-flag | Rapamycin regulated Shp2 phosphatase with mVenus and flag tag |
| 188656 | flag-RapR-PTP1B | Rapamycin regulated PTP1B with flag tag |
| 188657 | mVenus-RapR-PTPPest | Rapamycin regulated PTPPest with mVenus tag |
| 188658 | FRB-GFP-Gab1(Y628F/Y660F) | FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe |
| 188659 | FRB-GFP-Gab2(Y604F/Y634F) | FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe |
| 188660 | mVenus-FAK(397)FRB | mVenus tagged Focal Adhesion Kinase with FRB inserted at Y397 |