A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics.
Gassaway BM, Li J, Rad R, Mintseris J, Mohler K, Levy T, Aguiar M, Beausoleil SA, Paulo JA, Rinehart J, Huttlin EL, Gygi SP
Nat Methods. 2022 Oct 24. pii: 10.1038/s41592-022-01638-5. doi: 10.1038/s41592-022-01638-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188526 | iSPI_pSer_Subpool#1 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188527 | iSPI_pSer_Subpool#2 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188528 | iSPI_pSer_Subpool#3 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188529 | iSPI_pSer_Subpool#4 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188530 | iSPI_pSer_Subpool#5 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188531 | iSPI_pSer_Subpool#6 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188532 | iSPI_pSer_Subpool#7 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188533 | iSPI_pSer_Subpool#8 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188534 | iSPI_pSer_Subpool#9 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188535 | iSPI_pSer_Subpool#10 | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 188536 | iSPI_pSer_Full_library | Express a library of MBP-Fusion proteins containing phosphoserine at a defined position in the fusion protein |
| 192872 | rEcoli XpS | MG1655-C321 -- All 321 UAG codons changed to UAA |