Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries
(Pooled Library #188526, #188527, #188528, #188529, #188530, #188531, #188532, #188533, #188534, #188535, #188536, #1000000199, #1000000200, #1000000201, #1000000202, #1000000203, #1000000204, #1000000205, #1000000206, #1000000207, #1000000208, #1000000209)
-
Purpose
The purpose of this library is to generate human-derived phosphopeptides in E. coli using the phosphoserine orthogonal translation system for use as a phosphoproteomics resource. This library is a new generation of the human phosphoserine library published by Barber and Rinehart in Nature Biotechnology in 2018.
Each library is available individually or as a kit composed of:
- Library
- Bacterial strain 192872: rEcoli XpS
- Plasmid 188537: pSerOTS-C1* (V70)
-
Vector Backbone
-
Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Pooled Library | 188526 | iSPI_pSer_Subpool#1 | 1 | $430 | Add to Cart | |
Pooled Library | 188527 | iSPI_pSer_Subpool#2 | 1 | $430 | Add to Cart | |
Pooled Library | 188528 | iSPI_pSer_Subpool#3 | 1 | $430 | Add to Cart | |
Pooled Library | 188529 | iSPI_pSer_Subpool#4 | 1 | $430 | Add to Cart | |
Pooled Library | 188530 | iSPI_pSer_Subpool#5 | 1 | $430 | Add to Cart | |
Pooled Library | 188531 | iSPI_pSer_Subpool#6 | 1 | $430 | Add to Cart | |
Pooled Library | 188532 | iSPI_pSer_Subpool#7 | 1 | $430 | Add to Cart | |
Pooled Library | 188533 | iSPI_pSer_Subpool#8 | 1 | $430 | Add to Cart | |
Pooled Library | 188534 | iSPI_pSer_Subpool#9 | 1 | $430 | Add to Cart | |
Pooled Library | 188535 | iSPI_pSer_Subpool#10 | 1 | $430 | Add to Cart | |
Pooled Library | 188536 | iSPI_pSer_Full_library | 1 | $640 | Add to Cart | |
Pooled Library | 1000000199 | iSPI_pSer_Subpool#1 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000200 | iSPI_pSer_Subpool#2 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000201 | iSPI_pSer_Subpool#3 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000202 | iSPI_pSer_Subpool#4 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000203 | iSPI_pSer_Subpool#5 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000204 | iSPI_pSer_Subpool#6 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000205 | iSPI_pSer_Subpool#7 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000206 | iSPI_pSer_Subpool#8 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000207 | iSPI_pSer_Subpool#9 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000208 | iSPI_pSer_Subpool#10 Kit | 1 | $540 | Add to Cart | |
Pooled Library | 1000000209 | iSPI_pSer_Full_library Kit | 1 | $750 | Add to Cart |
Library Details
-
SpeciesEscherichia coli (E. coli)
-
No. plasmids188526-188535: ~11,000; 188536: ~110,139
-
Variable insert region144-188 bp
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
-
Volume∼10 µL
-
Concentration100 ng/µL
Resource Information
-
Protocols
-
Depositor Data
-
Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #1 Library was a gift from Jesse Rinehart (Addgene #188526)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #2 Library was a gift from Jesse Rinehart (Addgene #188527)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #3 Library was a gift from Jesse Rinehart (Addgene #188528)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #4 Library was a gift from Jesse Rinehart (Addgene #188529)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #5 Library was a gift from Jesse Rinehart (Addgene #188530)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #6 Library was a gift from Jesse Rinehart (Addgene #188531)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #7 Library was a gift from Jesse Rinehart (Addgene #188532)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #8 Library was a gift from Jesse Rinehart (Addgene #188533)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #9 Library was a gift from Jesse Rinehart (Addgene #188534)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #10 Library was a gift from Jesse Rinehart (Addgene #188535)
Iterative Synthetically Phosphorylated Isomers (iSPI) Full Library was a gift from Jesse Rinehart (Addgene #188536)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #1 Library Kit was a gift from Jesse Rinehart (Addgene #1000000199)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #2 Library Kit was a gift from Jesse Rinehart (Addgene #1000000200)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #3 Library Kit was a gift from Jesse Rinehart (Addgene #1000000201)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #4 Library Kit was a gift from Jesse Rinehart (Addgene #1000000202)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #5 Library Kit was a gift from Jesse Rinehart (Addgene #1000000203)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #6 Library Kit was a gift from Jesse Rinehart (Addgene #1000000204)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #7 Library Kit was a gift from Jesse Rinehart (Addgene #1000000205)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #8 Library Kit was a gift from Jesse Rinehart (Addgene #1000000206)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #9 Library Kit was a gift from Jesse Rinehart (Addgene #1000000207)
Iterative Synthetically Phosphorylated Isomers (iSPI) Subpool #10 Library Kit was a gift from Jesse Rinehart (Addgene #1000000208)
Iterative Synthetically Phosphorylated Isomers (iSPI) Full Library Kit was a gift from Jesse Rinehart (Addgene #1000000209) -
For your References section:
A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Gassaway BM, Li J, Rad R, Mintseris J, Mohler K, Levy T, Aguiar M, Beausoleil SA, Paulo JA, Rinehart J, Huttlin EL, Gygi SP. Nat Methods. 2022 Oct. doi: 10.1038/s41592-022-01638-5. PubMed 36280721