Disrupted expression of mitochondrial NCLX sensitizes neuroglial networks to excitotoxic stimuli and renders synaptic activity toxic.
Hagenston AM, Yan J, Bas-Orth C, Tan Y, Sekler I, Bading H
J Biol Chem. 2021 Dec 20:101508. doi: 10.1016/j.jbc.2021.101508.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181870 | pAAV-shNCLX-1 | Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter |
| 181871 | pAAV-shNCLX-2 | Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter |
| 181872 | pAAV-EGFP.T2A.NCLX.3'UTR | Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR) |
| 181873 | pAAV-EGFP.T2A.NCLX | Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter |
| 181874 | pAAV-4mtD3cpv | Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter |
| 181875 | pAAV-shCTRL | Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter |