Temporal dynamics of base excision/single-strand break repair protein complex assembly/disassembly are modulated by the PARP/NAD(+)/SIRT6 axis.
Koczor CA, Saville KM, Andrews JF, Clark J, Fang Q, Li J, Al-Rahahleh RQ, Ibrahim M, McClellan S, Makarov MV, Migaud ME, Sobol RW
Cell Rep. 2021 Nov 2;37(5):109917. doi: 10.1016/j.celrep.2021.109917.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176056 | pLV-CMV-EGFP-PolB-Hygro | EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette |
| 176062 | pLV-CMV-XRCC1-EGFP-Hygro | EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette |
| 176063 | pLV-EF1A-LivePAR-Hygro | EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette |
| 176064 | pUC19-POLBHR-eGFP | Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1 |
| 176065 | pLV-Hygro-EF1A-FHA-Linker-eGFP | EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette |
| 176066 | pLV-Hygro-EF1A-KR-Linker-eGFP | EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette |
| 176067 | pLV-Hygro-EF1A-Macro-Linker-eGFP | EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette |
| 176068 | pLV-Hygro-EF1A-OB-Linker-eGFP | EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette |
| 176069 | pLV-Hygro-EF1A-PBM-Linker-eGFP | EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette |
| 176070 | pLV-Hygro-EF1A-PBZC-Linker-eGFP | EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette |
| 176071 | pLV-Hygro-EF1A-RG-Linker-eGFP | EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette |
| 176072 | pLV-Hygro-EF1A-WWE-Linker-eGFP | EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette |
| 176073 | pLV-EF1A-LivePAR(Y107A)-Hygro | EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette |
| 176074 | pLV-Hygro-EF1A-BRCT1-Linker-eGFP | EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette |
| 176084 | pLV-Hygro-EF1A-XL1/BRCT1-Linker-eGFP | EGFP fused to the C-terminus of a XL1/BRCT1 domain & a hygromycin resistance cassette |
| 176085 | pLV-Hygro-EF1A-BRCT2-Linker-eGFP | EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette |
| 176086 | pLV-CMV-EGFP-PolB-PAMmut-Hygro | EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette |
| 176087 | pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro | EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette |
| 176089 | pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro | EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette |
| 176090 | pLentiCRISPRv2-POLB-KO-g1 | Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette |
| 176139 | pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut) | EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette |
| 176140 | pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut) | EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis |
| 176141 | pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut) | EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette |
| 176143 | pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A) | EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette |
| 176144 | pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A) | EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette |
| 176146 | pLV-Hygro-EF1A-EGFP-PARP1 | EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette |
| 176149 | pLVX-CMV-XRCC1-gRNA res-Neo | XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette |
| 176150 | pLV-Hygro-Ef1A-Myc-POLB(PAMmut) | N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette |
| 176526 | pLV-Hygro-EF1A-LivePARBackbone | Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR |