CRISPR-Mediated Transcriptional Repression in Toxoplasma gondii.
Markus BM, Boydston EA, Lourido S
mSphere. 2021 Oct 13:e0047421. doi: 10.1128/mSphere.00474-21.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171088 | pU6-Universal.Sth.dCas9 | CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites. |
| 171089 | pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy) | CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines. |
| 171090 | pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP | CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines. |