Michael Ward Lab: Characterization of iPSC lines for the iPSC Neurodegenerative Disease Initiative (iNDI)

Unpublished

Please see Addgene's curated iPSC Neurodegenerative Disease Initiative (iNDI) Collection page for additional information about the collection.

Plasmids from Article

ID	Plasmid	Purpose
182306	PB-TO-NFIA.1-SOX9 (bi-directional promoter)	Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression
182307	PB-TO-ASCL1-DLX2	Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
182308	PB-TO-hNGN2-BSD-mApple	Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple
182309	PB-TO-MYOD1-shOct4	Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression
182310	PB-TO-NFIA.1-SOX9	Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression
182311	PB-TO-NGN2-EMX1 BSD-mApple	Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple
182312	PB-TO-NGN2-EMX1 puro-BFP	Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, puromycin selection, BFP
182313	PB-TO-hNIL BSD-mApple	Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple
198397	PB-TO-oNGN2	Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
204733	iC13 PB-TO-oNgn2-BFP-puro	Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.
204734	iF59 PB-TO-oNgn2-puro	Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.