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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complex.

Gerbracht JV, Boehm V, Britto-Borges T, Kallabis S, Wiederstein JL, Ciriello S, Aschemeier DU, Kruger M, Frese CK, Altmuller J, Dieterich C, Gehring NH
Nucleic Acids Res. 2020 Jul 4. pii: 5867419. doi: 10.1093/nar/gkaa564. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
158540PB-CuO-V5 CASC3 siRes1+2 f.l. WTFor PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3
158541PB-CuO-V5 CASC3 siRes 1-480 WTFor PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480
158542PB-CuO-V5 CASC3 siRes 1-480 188/218-mutFor PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480
158543PB-CuO-V5 CASC3 siRes 110-480 WTFor PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480
158544PB-CuO-V5-CASC3 siRes 110-480 188/218-mutFor PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480

Antibodies from Article