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Molecular and functional interaction between protocadherin-gammaC5 and GABAA receptors.

Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali R, Chen WV, Maniatis T, De Blas AL
J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 10.1523/JNEUROSCI.0969-12.2012. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
119933PCDHGC5-EGFPProtocadherin gamma C5 expression (EGFP-tag at the C-terminus).
119934PCDHGC5-mCherryProtocadherin gamma C5 expression (mCherry-tag at the C-terminus).
119935Myc-PCDHGC5Protocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)
122227PCDHGC5 sh1 shRNAKnock Down Protocadherin gamma C5. It targets Protocadherin gamma C5 mRNA (nucleotides 851–871 in the protein coding region).
122228PCDHGC5 sh1 3m shRNANegative control shRNA (with 3-point mutations), for plasmid 122227: targeting Protocadherin gamma C5 mRNA.
122229Rescue myc-PCDHGC5 mRNARescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.

Antibodies from Article