Rescue myc-PCDHGC5 mRNA
(Plasmid #122229)

• Purpose: Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.
• Depositing Lab: Angel de Blas
• Publication: Li et al J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 10.1523/JNEUROSCI.0969-12.2012.

Backbone

• Vector backbone: pCDNA 3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCDHGC5
• Species: R. norvegicus (rat)
• Mutation: Silent mutations are in five consecutive codons (ACATCTGAAGCAGTG to ACGTCCGAGGCTGTTboth encoding TSEAV) in the PCDHGC5 sh1 shRNA target site
• Entrez Gene: Pcdhgc5
• Tag / Fusion Protein:
  • 9E10 cMyc epitope (EQKLISEEDL) was inserted between amino acids 103 and 104 of mature protein (after signal peptide cleavage) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert was originally cloned in Angel de Blas's lab.