Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon.
Messier JE, Chen H, Cai ZL, Xue M
Elife. 2018 Aug 9;7. pii: 38506. doi: 10.7554/eLife.38506.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 114367 | pCAG-hChR2(H134R)-EYFP | expresses channelrhodopsin 2 fused with EYFP |
| 114368 | pAAV-EF1α-DIO-ReaChR-P2A-dTomato | expresses a Cre-dependent red light-shifted channelrhodopsin variant |
| 114369 | pAAV-EF1α-FRT-FLEX-GtACR2-EYFP | expresses Flpo recombinase-dependent GtACR2 |
| 114370 | pAAV-EF1α-FRT-FLEX-GtACR1-EYFP | expresses Flpo recombinase-dependent GtACR1 |
| 114371 | pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD | expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment |
| 114372 | pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C | expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment |
| 114373 | pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL | expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment |
| 114374 | pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC | expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment |
| 114375 | pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C | expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment |
| 114376 | pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC | expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment |
| 114377 | pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC | expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting |
| 114378 | pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP | expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment |