Engineering extrinsic disorder to control protein activity in living cells.
Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, Dokholyan NV, Hahn KM
Science. 2016 Dec 16;354(6318):1441-1444.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86974 | mCherry-PI(WT)-Vav2(DPZ) | PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2 |
| 86977 | mCherry-PA(WT)-Vav2 | PI(DM): Photo-activatable/light sensitive |
| 86978 | pTriex-mVenus-PI-RhoA(Q63L) | PI(DM): Photo-activatable/light sensitive |
| 87356 | pBabe-PI(WT)-Src(WT)-mCherry | PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type |
| 87357 | pBabe-PI(WT)-Src(YF)-mCherry | PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation |
| 87358 | pBabe-PI(WT)-Src(KD)-mCherry | PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation |
| 87550 | pBabe-PI(WT)-Rac1(WT)-mCherry | PI(WT): Photo-inhibitable/blue light sensitive Rac1 |
| 87553 | pBabe-PI(WT)-Rac1(Q61L)-mCherry | PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1 |
| 87589 | PI(DM)-Rac1(WT)dc1.FLARE | PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor |
| 87590 | PI(LM)-Rac1(WT)dc1.FLARE | PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor |
| 91873 | pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2 | Photo-activatable/light sensitive Vav2 |
| 91874 | pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ) | Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 |
| 91875 | mCherry-uniRapR-P-Vav2(DPZ) | Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 |
| 91876 | pBabe-tetoff-mVenus-PICdc42 | PI(WT): Photo inhibitable (blue light sensitive) |